Active Recombinant Full Length Human HYAL1 Protein, C-6×His tagged

• Cat.No.: HYAL1-11HFL
• Product Overview: Recombinant Full Length Human HYAL1 Protein with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: CHO
• Species: Human
• Tag: C-6×His
• Bio-activity: Measured by its ability to hydrolyze S-35 labeled hyaluronan. < 20 ng of rhHYAL1 is required for the 50 % hydrolysis of 10 μg hyaluronan, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 47 kDa
  SDS-PAGE: 50-60 kDa, reducing conditions
• N-terminal Sequence Analysis: Phe22
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 90 %, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 μg per lane
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: HYAL1 hyaluronidase 1 [ Homo sapiens (human) ]
• Official Symbol: HYAL1
• Synonyms: HYAL1; hyaluronidase 1; MPS9; NAT6; LUCA1; HYAL-1; hyaluronidase-1; hyaluronoglucosaminidase 1; luCa-1; lung carcinoma protein 1; plasma hyaluronidase; tumor suppressor LUCA-1; EC 3.2.1.35
• Gene ID: 3373
• mRNA Refseq: NM_033159
• Protein Refseq: NP_149349
• MIM: 607071
• UniProt ID: Q12794