Active Recombinant Full Length Human IKBKE Protein, N-Flag,GST-tagged

• Cat.No.: IKBKE-80HFL
• Product Overview: N-terminal FLAG and GST-tagged, recombinant, human IKKε full length, expressed by baculovirus in Sf21 insect cells. Purified by immunoaffinity chromatography.

Specification

• Description: IKKe is a non-canonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
• Source: Sf21
• Species: Human
• Tag: Flag,GST
• Protein length: Full Length
• Form: 0.142mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 805U/mg, where one unit of IKK, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 109 kDa
• AA Sequence: 1 MDYKDDDDKS PILGYWKIKG LVQPTRLLLE YLEEKYEEHL YERDEGDKWR NKKFELGLEF 61 PNLPYYIDGD VKLTQSMAII RYIADKHNML GGCPKERAEI SMLEGAVLDI RYGVSRIAYS 121 KDFETLKVDF LSKLPEMLKM FEDRLCHKTY LNGDHVTHPD FMLYDALDVV LYMDPMCLDA 181 FPKLVCFKKR IEAIPQIDKY LKSSKYIAWP LQGWQATFGG GDHPPKSDLV PRGSHNQTSL 241 YKKAGTMQST ANYLWHTDDL LGQGATASVY KARNKKSGEL VAVKVFNTTS YLRPREVQVR 301 EFEVLRKLNH QNIVKLFAVE ETGGSRQKVL VMEYCSSGSL LSVLESPENA FGLPEDEFLV 361 VLRCVVAGMN HLRENGIVHR DIKPGNIMRL VGEEGQSIYK LTDFGAAREL DDDEKFVSVY 421 GTEEYLHPDM YERAVLRKPQ QKAFGVTVDL WSIGVTLYHA ATGSLPFIPF GGPRRNKEIM 481 YRITTEKPAG AIAGAQRREN GPLEWSYTLP ITCQLSLGLQ SQLVPILANI LEVEQAKCWG 541 FDQFFAETSD ILQRVVVHVF SLSQAVLHHI YIHAHNTIAI FQEAVHKQTS VAPRHQEYLF 601 EGHLCVLEPS VSAQHIAHTT ASSPLTLFST AIPKGLAFRD PALDVPKFVP KVDLQADYNT 661 AKGVLGAGYQ ALRLARALLD GQELMFRGLH WVMEVLQATC RRTLEVARTS LLYLSSSLGT 721 ERFSSVAGTP EIQELKAAAE LRSRLRTLAE VLSRCSQNIT ETQESLSSLN RELVKSRDQV 781 HEDRSIQQIQ CCLDKMNFIY KQFKKSRMRP GLGYNEEQIH KLDKVNFSHL AKRLLQVFQE 841 ECVQKYQASL VTHGKRMRVV HETRNHLRLV GCSVAACNTE AQGVQESLSK LLEELSHQLL 901 QDRAKGAQAS PPPIAPYPSP TRKDLLLHMQ ELCEGMKLLA SDLLDNNRII ERLNRVPAPP 961 DV
• Purity: Purity 84.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: IKBKE inhibitor of nuclear factor kappa B kinase subunit epsilon [ Homo sapiens (human) ]
• Official Symbol: IKBKE
• Synonyms: IKBKE; inhibitor of nuclear factor kappa B kinase subunit epsilon; IKKE; IKKI; IKK-E; IKK-i
• Gene ID: 9641
• mRNA Refseq: NM_014002.4
• Protein Refseq: NP_054721.1
• MIM: 605048
• UniProt ID: Q14164