Active Recombinant Full Length Human ITK Protein, GST-tagged

• Cat.No.: ITK-2292HF
• Product Overview: Full-length human ITK [2-620(end) amino acids of accession number NP_005537.3] was expressed as N- terminal GST-fusion protein (99 kDa) using baculovirus expression system. GST-ITK was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 2-620(end)
• Purity: 85%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: IL2-inducible T-cell kinase
• Official Symbol: ITK
• Synonyms: ITK;EMT;LPFS1;LYK;PSCTK2
• Protein Refseq: NP_005537.3
• UniProt ID: Q08881