Active Recombinant Full Length Human KMO Protein, C-Flag-tagged

• Cat.No.: KMO-246HFL
• Product Overview: Recombinant Full Length Human KMO Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: The specific activity of KMO was determined by measuring the product 3-hydroxykynurenine formation from a conversion of Kynurenine. The reaction was carried out at 37 centigrade for 40 min in the buffer containing 100 mM Tris, pH8.0, 10 mM KCl, 1 mM NADPH, 3 mM glucose-6-phos-phate, 1 units/ml of glucose-6 phosphate dehydrogenase, and 100 ?M kynurenine as the substrate Surface Plasmon Ressonance (SPR).
• Molecular Mass: 55.6 kDa
• AA Sequence: MDSSVIQRKKVAVIGGGLVGSLQAC FLAKRNFQIDVYEAREDTRVATFTR GRSINLALSHRGRQALKAVG LEDQIVSQGIPMRARMIHSLSGKKS AIPYGTKSQYILSVSRENLNKDLLT AAEKYPNVKMHFNHRLLKCN PEEGMITVLGSDKVPKDVTCDLIVG CDGAYSTVRSHLMKKPRFDYSQQYI PHGYMELTIPPKNGDYAMEP NYLHIWPRNTFMMIALPNMNKSFTC TLFMPFEEFEKLLTSNDVVDFFQKY FPDAIPLIGEKLLVQDFFLL PAQPMISVKCSSFHFKSHCVLLGDA AHAIVPFFGQGMNAGFEDCLVFDEL MDKFSNDLSLCLPVFSRLRI PDDHAISDLSMYNYIEMRAHVNSSW FIFQKNMERFLHAIMPSTFIPLYTM VTFSRIRYHEAVQRWHWQKK VINKGLFFLGSLIAISSTYLLIHYM SPRSFLCLRRPWNWIAHFRNTTCFP AKAVDSLEQISNLISRTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism

Gene Information

• Gene Name: KMO kynurenine 3-monooxygenase [ Homo sapiens (human) ]
• Official Symbol: KMO
• Synonyms: dJ317G22.1
• Gene ID: 8564
• mRNA Refseq: NM_003679.5
• Protein Refseq: NP_003670.2
• MIM: 603538
• UniProt ID: O15229