Active Recombinant Full Length Human LYN Protein, N-His-tagged
Cat.No. : | LYN-95HFL |
Product Overview : | N-terminal 6His-tagged recombinant full length human Lyn expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose. |
- Specification
- Gene Information
- Related Products
Description : | The Lyn protein is a member of the Src family of non-receptor-associated protein-tyrosine kinases. LYN A differs from LYN B in having an additional 21 amino acids at the amino terminus. Lyn undergoes a concentration-dependent autophosphorylation, thus activating the catalytic site and decreasing the accessibility of the SH2 domain. Lyn phosphorylates and activates Tec, non-receptor tyrosine kinase that is coexpressed with Lyn and acts downstream of Lyn in intracellular signaling pathways. Lyn tyrosine kinase has been shown to be necessary for IgE-receptor (FceRI)-mediated mast-cell activation. |
Source : | Sf21 |
Species : | Human |
Tag : | His |
Protein length : | Full Length |
Form : | 0.889mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : | 1140U/mg, where one unit of Lyn activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : | 59.6 kDa |
AA Sequence : | 1 MHHHHHHMGC IKSKGKDSLS DDGVDLKTQP VRNTERTIYV RDPTSNKQQR PVPESQLLPG 61 QRFQTKDPEE QGDIVVALYP YDGIHPDDLS FKKGEKMKVL EEHGEWWKAK SLLTKKEGFI 121 PSNYVAKLNT LETEEWFFKD ITRKDAERQL LAPGNSAGAF LIRESETLKG SFSLSVRDFD 181 PVHGDVIKHY KIRSLDNGGY YISPRITFPC ISDMIKHYQK QADGLCRRLE KACISPKPQK 241 PWDKDAWEIP RESIKLVKRL GAGQFGEVWM GYYNNSTKVA VKTLKPGTMS VQAFLEEANL 301 MKTLQHDKLV RLYAVVTREE PIYIITEYMA KGSLLDFLKS DEGGKVLLPK LIDFSAQIAE 361 GMAYIERKNY IHRDLRAANV LVSESLMCKI ADFGLARVIE DNEYTAREGA KFPIKWTAPE 421 AINFGCFTIK SDVWSFGILL YEIVTYGKIP YPGRTNADVM TALSQGYRMP RVENCPDELY 481 DIMKMCWKEK AEERPTFDYL QSVLDDFYTA TEGQYQQQP |
Purity : | Purity 94.7% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 6 months at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | LYN LYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ] |
Official Symbol : | LYN |
Synonyms : | LYN; LYN proto-oncogene, Src family tyrosine kinase; JTK8; SAIDV; p53Lyn; p56Lyn |
Gene ID : | 4067 |
mRNA Refseq : | NM_002350.4 |
Protein Refseq : | NP_002341.1 |
MIM : | 165120 |
UniProt ID : | P07948 |
Products Types
◆ Recombinant Protein | ||
LYN-1343H | Recombinant Human LYN Protein, MYC/DDK-tagged | +Inquiry |
LYN-4556H | Recombinant Human LYN Protein, GST-tagged | +Inquiry |
LYN-12H | Recombinant Human LYN Protein (Full Length), N-FLAG tagged | +Inquiry |
LYN-514H | Recombinant Human LYN Protein, His-tagged | +Inquiry |
Lyn-3877M | Recombinant Mouse Lyn Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
LYN-656HCL | Recombinant Human LYN cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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