| Description : |
c-Jun N-terminal kinases (JNKs), a stress-activated protein kinase (SAPK), is involved in cellular responses to environmental stresses. JNK kinases can be activated by stimuli such as UV light, radiation, protein synthesis inhibitors, ceramide, DNA-damaging drugs, chemopreventive drugs, TNF-a, and interleukin 1. JNK binds to an NH2-terminal region of ATF2 and c-Jun and phosphorylates two sites within the activation domain of each transcription factor. Three JNK genes (Jnk1, -2 and -3) have been identified in humans; however, splice variants result in a total of 10 isoforms. Inhibition of JNK3 (MAPK10, Mitogen-activated protein kinase 10, Stress-activated protein kinase JNK3) may provide clinical benefit in diseases as diverse as immune inflammatory (joint, bowel, pulmonary), ischemic injury (brain, cardiac) and neurodegenerative (Alzheimer's, Parkinson's) diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.76mg/ml of 50mM Tris/HCl, pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : |
128U/mg, where one unit of JNK3 activity is defined as 1nmol phosphate incorporated into 3μM ATF-2 (cat# 12-367) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
53 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMGSPG ISGVDVSSVA KHYNMSKSKV DNQFYSVEVG 61 DSTFTVLKRY QNLKPIGSGA QGIVCAAYDA VLDRNVAIKK LSRPFQNQTH AKRAYRELVL 121 MKCVNHKNII SLLNVFTPQK TLEEFQDVYL VMELMDANLC QVIQMELDHE RMSYLLYQML 181 CGIKHLHSAG IIHRDLKPSN IVVKSDCTLK ILDFGLARTA GTSFMMTPYV VTRYYRAPEV 241 ILGMGYKENV DIWSVGCIMG EMVRHKILFP GRDYIDQWNK VIEQLGTPCP EFMKKLQPTV 301 RNYVENRPKY AGLTFPKLFP DSLFPADSEH NKLKASQARD LLSKMLVIDP AKRISVDDAL 361 QHPYINVWYD PAEVEAPPPQ IYDKQLDERE HTIEEWKELI YKEVMNSEEK TKNGVVKGQP 421 SPSGAAVNSS ESLPPSSSVN DISSMSTDQT LASDTDSSLE ASAGPLGCCR |
| Purity : |
Purity 99% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at - 70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
