| Description : |
Mitogen-activated protein (MAP) kinases are proline-directed protein kinases that mediate the effects of numerous extracellular stimuli on a wide array of biological processes, such as cellular proliferation, differentiation, and death. Three groups of mammalian MAP kinases have been studied in detail: the extracellular signal-regulated kinases (ERK), the c-Jun NH2-terminal kinases (JNK), and the p38 MAP kinases. The p38 MAP kinase group, which includes the isoforms p38a, p38b, p38g and p38d, activates the transcription factors CREB, ATF1, ATF2, CHOP, and MEF-2C. The p38 MAP kinase also activates other protein kinases, such as Mapkap-2, Mapkap-3, and Mnk1/2. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
Full Length |
| Form : |
1.59mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Liquid solution at -20 centigrade. |
| Bio-activity : |
24U/mg, where one unit of SAPK2a, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
67.7 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMSQE RPTFYRQELN 241 KTIWEVPERY QNLSPVGSGA YGSVCAAFDT KTGLRVAVKK LSRPFQSIIH AKRTYRELRL 301 LKHMKHENVI GLLDVFTPAR SLEEFNDVYL VTHLMGADLN NIVKCQKLTD DHVQFLIYQI 361 LRGLKYIHSA DIIHRDLKPS NLAVNEDCEL KILDFGLARH TDDEMTGYVA TRWYRAPEIM 421 LNWMHYNQTV DIWSVGCIMA ELLTGRTLFP GTDHIDQLKL ILRLVGTPGA ELLKKISSES 481 ARNYIQSLTQ MPKMNFANVF IGANPLAVDL LEKMLVLDSD KRITAAQALA HAYFAQYHDP 541 DDEPVADPYD QSFESRDLLI DEWKSLTYDE VISFVPPPLD QEEMES |
| Purity : |
Purity 98% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
