Active Recombinant Full Length Human MAPK14(T106M) Mutation Protein, N-GST-tagged

• Cat.No.: MAPK14-119HFL
• Product Overview: N-terminal GST-tagged, recombinant, human, full-length p38α/SAPK2a containing the mutation T106M. Expressed in E. coli cells. Purified using glutathione-agarose. Activated using DD-MKK6 and repurified using glutathione- agarose.

Specification

• Description: N-terminal GST-tagged recombinant, human full length p38α/SAPK2a containing the mutation T106M. Activated using DD-MKK6 (14-537) and repurified using glutathione-agarose. Contains the gatekeeper mutation T160M in the ATP-binding domain. This mutation increases resistance to SB203580 as compared to the wild type sequence.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 1.879mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Liquid solution at -20 centigrade.
• Bio-activity: 97U/mg, where one unit of p38α/SAPK2a (T106M) activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.7 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMSQE RPTFYRQELN 241 KTIWEVPERY QNLSPVGSGA YGSVCAAFDT KTGLRVAVKK LSRPFQSIIH AKRTYRELRL 301 LKHMKHENVI GLLDVFTPAR SLEEFNDVYL VMHLMGADLN NIVKCQKLTD DHVQFLIYQI 361 LRGLKYIHSA DIIHRDLKPS NLAVNEDCEL KILDFGLARH TDDEMTGYVA TRWYRAPEIM 421 LNWMHYNQTV DIWSVGCIMA ELLTGRTLFP GTDHIDQLKL ILRLVGTPGA ELLKKISSES 481 ARNYIQSLTQ MPKMNFANVF IGANPLAVDL LEKMLVLDSD KRITAAQALA HAYFAQYHDP 541 DDEPVADPYD QSFESRDLLI DEWKSLTYDE VISFVPPPLD QEEMES
• Purity: Purity 92.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAPK14 mitogen-activated protein kinase 14 [ Homo sapiens (human) ]
• Official Symbol: MAPK14
• Synonyms: MAPK14; mitogen-activated protein kinase 14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• Gene ID: 1432
• mRNA Refseq: NM_001315.3
• Protein Refseq: NP_001306.1
• MIM: 600289
• UniProt ID: Q16539