Active Recombinant Full Length Human MATK Protein, C-His-tagged

• Cat.No.: MATK-103HFL
• Product Overview: C-terminal 6His-tagged, recombinant, human MATK full length expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: MATK (Megakaryocyte-associated tyrosine kinase) is a non-receptor protein tyrosine kinase that belongs to the Src family of kinases. It is predominantly expressed in hematopoietic cells, particularly in megakaryocytes, platelets, and B cells. Research has suggested that MATK may be involved in the development and progression of several types of cancer, including breast cancer, leukemia, and lymphoma. In breast cancer, MATK has been shown to be upregulated in a subset of tumors, and may promote cancer cell growth and survival. In leukemia and lymphoma, MATK has been shown to be involved in the regulation of cell survival and proliferation, and may be a potential therapeutic target for the treatment of these diseases.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.86 mg/ml of enzyme in 20 mM Hepes/NaOH pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5 mM EGTA, 5 mM DTT, 50% glycerol. Frozen solution.
• Bio-activity: 15 U/mg, where one unit of MATK, active activity is defined as 1 nmol phosphate incorporated into 250 μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 53 kDa
• Purity: 75% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MATK megakaryocyte-associated tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: MATK
• Synonyms: MATK; megakaryocyte-associated tyrosine kinase; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK
• Gene ID: 4145
• mRNA Refseq: NM_139354.3
• Protein Refseq: NP_647611.1
• MIM: 600038
• UniProt ID: P42679