Active Recombinant Full Length Human NCF1 Protein, C-Flag-tagged

• Cat.No.: NCF1-576HFL
• Product Overview: Recombinant Full Length Human NCF1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme substrate
• Molecular Mass: 44.5 kDa
• AA Sequence: MGDTFIRHIALLGFEKRFVPSQHYV YMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENR IIPHLPAPKWFDGQRAAENRQGTLT EYCSTLMSLPTKISRCPHLLDFFKV RPDDLKLPTDNQTKKPETYL MPKDGKSTATDITGPIILQTYRAIA NYEKTSGSEMALSTGDVVEVVEKSE SGWWFCQMKAKRGWIPASFL EPLDSPDETEDPEPNYAGEPYVAIK AYTAVEGDEVSLLEGEAVEVIHKLL DGWWVIRKDDVTGYFPSMYL QKSGQDVSQAQRQIKRGAPPRRSSI RNAHSIHQRSRKRLSQDAYRRNSVR FLQQRRRQARPGPQSPGSPL
  EEERQTQRSKPQPAVPPRPSADLIL NRCSESTKRKLASPVTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton

Gene Information

• Gene Name: NCF1 neutrophil cytosolic factor 1 [ Homo sapiens (human) ]
• Official Symbol: NCF1
• Synonyms: CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Gene ID: 653361
• mRNA Refseq: NM_000265.7
• Protein Refseq: NP_000256.4
• MIM: 608512
• UniProt ID: P14598