Active Recombinant Full Length Human PCBP2 Protein, C-Flag-tagged

• Cat.No.: PCBP2-412HFL
• Product Overview: Recombinant Full Length Human PCBP2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 38.5 kDa
• AA Sequence: MDTGVIEGGLNVTLTIRLLMHGKEV GSIIGKKGESVKKMREESGARINIS EGNCPERIITLAGPTNAIFK AFAMIIDKLEEDISSSMTNSTAASR PPVTLRLVVPASQCGSLIGKGGCKI KEIRESTGAQVQVAGDMLPN STERAITIAGIPQSIIECVKQICVV MLETLSQSPPKGVTIPYRPKPSSSP VIFAGGQDRYSTGSDSASFP HTTPSMCLNPDLEGPPLEAYTIQGQ YAIPQPDLTKLHQLAMQQSHFPMTH GNTGFSGIESSSPEVKGYWA GLDASAQTTSHELTIPNDLIGCIIG RQGAKINEIRQMSGAQIKIANPVEG STDRQVTITGSAASISLAQY
  LINVRLSSETGGMGSSTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PCBP2 poly(rC) binding protein 2 [ Homo sapiens (human) ]
• Official Symbol: PCBP2
• Synonyms: HNRPE2; HNRNPE2; hnRNP-E2
• Gene ID: 5094
• mRNA Refseq: NM_005016.6
• Protein Refseq: NP_005007.2
• MIM: 601210
• UniProt ID: Q15366