Active Recombinant Full Length Human PDK4 Protein, N-GST-tagged

• Cat.No.: PDK4-126HFL
• Product Overview: N-terminal GST-tagged, recombinant, human PDHK4 full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Pyruvate dehydrogenase kinase isoenzyme 4 (PDHK4) is a member of the PDK/BCKDK protein kinase family. PDHK4 plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDHK4 is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.50 mg/ml of enzyme in 50 mM Tris/HCl pH 8.0, 4 mM glutathione, 266 mM NaCl, 0.09 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.9 mM benzamidine, 0.2 mM PMSF, 0.09% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 11 U/mg, where one unit of PDHK4 activity is defined as 1 nmol phosphate incorporated into 250 μM KKKYHGHSMSDPGVSYRT per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 74 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMKAAR 241 FVLRSAGSLN GAGLVPREVE HFSRYSPSPL SMKQLLDFGS ENACERTSFA FLRQELPVRL 301 ANILKEIDIL PTQLVNTSSV QLVKSWYIQS LMDLVEFHEK SPDDQKALSD FVDTLIKVRN 361 RHHNVVPTMA QGIIEYKDAC TVDPVTNQNL QYFLDRFYMN RISTRMLMNQ HILIFSDSQT 421 GNPSHIGSID PNCDVVAVVQ DAFECSRMLC DQYYLSSPEL KLTQVNGKFP DQPIHIVYVP 481 SHLHHMLFEL FKNAMRATVE HQENQPSLTP IEVIVVLGKE DLTIKISDRG GGVPLRIIDR 541 LFSYTYSTAP TPVMDNSRNA PLAGFGYGLP ISRLYAKYFQ GDLNLYSLSG YGTDAIIYLK 601 ALSSESIEKL PVFNKSAFKH YQMSSEADDW CIPSREPKNL AKEVAM
• Purity: Purity 80% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PDK4 pyruvate dehydrogenase kinase 4 [ Homo sapiens (human) ]
• Official Symbol: PDK4
• Synonyms: PDK4; pyruvate dehydrogenase kinase 4; Pyruvate Dehydrogenase Kinase 4; Pyruvate Dehydrogenase Kinase; Isozyme 4
• Gene ID: 5166
• mRNA Refseq: NM_002612.4
• Protein Refseq: NP_002603.1
• MIM: 602527
• UniProt ID: Q16654