Active Recombinant Full Length Human PIK3CG Protein, N-His-tagged

• Cat.No.: PIK3CG-138HFL
• Product Overview: N-terminal 6His-tagged recombinant full-length human PI3 kinase (p120γ) expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: PI3 Kinase (p120γ) belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. GenBank AF327656. The recombinant protein contains the amino acid substitution R459Q with respect to this accession number. This conflict is reported in GenBank XM_027912. The residue coordinates in the native sequence are given.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 34U/mg, where one unit of PI3 Kinase (p120γ) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate formed per minute at 22 centigrade with a final ATP concentration of 100μM.
• Bio-activity: 34U/mg, where one unit of PI3 Kinase (p120γ) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate formed per minute at 22 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 130 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSGI RPCMELENYK QPVVLREDNC RRRRRMKPRS 61 AAASLSSMEL IPIEFVLPTS QRKCKSPETA LLHVAGHGNV EQMKAQVWLR ALETSVAADF 121 YHRLGPHHFL LLYQKKGQWY EIYDKYQVVQ TLDCLRYWKA THRSPGQIHL VQRHPPSEES 181 QAFQRQLTAL IGYDVTDVSN VHDDELEFTR RGLVTPRMAE VASRDPKLYA MHPWVTSKPL 241 PEYLWKKIAN NCIFIVIHRS TTSQTIKVSP DDTPGAILQS FFTKMAKKKS LMDIPESQSE 301 QDFVLRVCGR DEYLVGETPI KNFQWVRHCL KNGEEIHVVL DTPPDPALDE VRKEEWPLVD 361 DCTGVTGYHE QLTIHGKDHE SVFTVSLWDC DRKFRVKIRG IDIPVLPRNT DLTVFVEANI 421 QHGQQVLCQR RTSPKPFTEE VLWNVWLEFS IKIKDLPKGA LLNLQIYCGK APALSSKASA 481 ESPSSESKGK VQLLYYVNLL LIDHRFLLRR GEYVLHMWQI SGKGEDQGSF NADKLTSATN 541 PDKENSMSIS ILLDNYCHPI ALPKHQPTPD PEGDRVRAEM PNQLRKQLEA IIATDPLNPL 601 TAEDKELLWH FRYESLKHPK AYPKLFSSVK WGQQEIVAKT YQLLARREVW DQSALDVGLT 661 MQLLDCNFSD ENVRAIAVQK LESLEDDDVL HYLLQLVQAV KFEPYHDSAL ARFLLKRGLR 721 NKRIGHFLFW FLRSEIAQSR HYQQRFAVIL EAYLRGCGTA MLHDFTQQVQ VIEMLQKVTL 781 DIKSLSAEKY DVSSQVISQL KQKLENLQNS QLPESFRVPY DPGLKAGALA IEKCKVMASK 841 KKPLWLEFKC ADPTALSNET IGIIFKHGDD LRQDMLILQI LRIMESIWET ESLDLCLLPY 901 GCISTGDKIG MIEIVKDATT IAKIQQSTVG NTGAFKDEVL NHWLKEKSPT EEKFQAAVER 961 FVYSCAGYCV ATFVLGIGDR HNDNIMITET GNLFHIDFGH ILGNYKSFLG INKERVPFVL 1021 TPDFLFVMGT SGKKTSPHFQ KFQDICVKAY LALRHHTNLL IILFSMMLMT GMPQLTSKED 1081 IEYIRDALTV GKNEEDAKKY FLDQIEVCRD KGWTVQFNWF LHLVLGIKQG EKHSA
• Purity: Purity 92.4% by SDS-PAGE and Coomassie blue staining. MW = 130kDa.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIK3CG phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma [ Homo sapiens (human) ]
• Official Symbol: PIK3CG
• Synonyms: PIK3CG; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; PI3K; PIK3; IMD97; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
• Gene ID: 5294
• mRNA Refseq: NM_002649.3
• Protein Refseq: NP_002640.2
• MIM: 601232
• UniProt ID: P48736