| Description : |
PIP5K1α (Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha) is a lipid kinase that phosphorylates phosphatidylinositol-4-phosphate (PI4P) to generate phosphatidylinositol 4,5-bisphosphate (PIP2) in the plasma membrane. PIP2 is an important signaling molecule that regulates a variety of cellular processes, including cytoskeletal dynamics, ion channel activity, and membrane trafficking. Research has suggested that PIP5K1α plays a role in several diseases, including cancer, inflammatory disorders, and neurological disorders. It has been shown to promote cancer cell proliferation and invasion, and inhibition of PIP5K1α has been suggested as a potential cancer therapy. Additionally, PIP5K1α has been implicated in the regulation of immune cell function, making it a potential target for the treatment of autoimmune diseases and inflammatory disorders. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.466mg/ml of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
11971U/mg, where one unit of PIP5K1α, active activity is defined as 1nmol phosphatidylinositol 4,5-bisphosphate (PIP2) formed per minute at room temperature with a final ATP concentration of 200μM. |
| Molecular Mass : |
60 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF AAKMASASSG PSSSVGFSSF DPAVPSCTLS 61 SASGIKRPMA SEVPYASGMP IKKIGHRSVD SSGETTYKKT TSSALKGAIQ LGITHTVGSL 121 STKPERDVLM QDFYVVESIF FPSEGSNLTP AHHYNDFRFK TYAPVAFRYF RELFGIRPDD 181 YLYSLCSEPL IELCSSGASG SLFYVSSDDE FIIKTVQHKE AEFLQKLLPG YYMNLNQNPR 241 TLLPKFYGLY CVQAGGKNIR IVVMNNLLPR SVKMHIKYDL KGSTYKRRAS QKEREKPLPT 301 FKDLDFLQDI PDGLFLDADM YNALCKTLQR DCLVLQSFKI MDYSLLMSIH NIDHAQREPL 361 SSETQYSVDT RRPAPQKALY STAMESIQGE ARRGGTMETD DHMGGIPARN SKGERLLLYI 421 GIIDILQSYR FVKKLEHSWK ALVHDGDTVS VHRPGFYAER FQRFMCNTVF KKIPCVHLGR 481 PDVLPQTPPL EEISEGSPIP DPSFSPLVGE TLQMLTTSTT LEKLEVAESE FTH |
| Purity : |
Purity 73.6% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
