Active Recombinant Full Length Human POLR2M Protein, C-Flag-tagged

• Cat.No.: POLR2M-511HFL
• Product Overview: Recombinant Full Length Human POLR2M Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a subunit of a specific form of RNA polymerase II termed Pol II(G). The encoded protein may act as a negative regulator of transcriptional activation by the Mediator complex. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 4. Readthrough transcription between this gene and the neighboring upstream gene MYZAP (myocardial zonula adherens protein) is represented with GeneID 145781.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: WB positive control
• Molecular Mass: 41.6 kDa
• AA Sequence: MCSLPRGFEPQAPEDLAQRSLVELR EMLKRQERLLRNEKFICKLPDKGKK IFDSFAKLKAAIAECEEVRR KSELFNPVSLDCKLRQKAIAEVDVG TDKAQNSDPILDTSSLVPGCSSVDN IKSSQTSQNQGLGRPTLEGD EETSEVEYTVNKGPASSNRDRVPPS SEASEHHPRHRVSSQAEDTSSSFDN LFIDRLQRITIADQGEQQSE ENASTKNLTGLSSGTEKKPHYMEVL EMRAKNPVPQLRKFKTNVLPFRQND SSSHCQKSGSPISSEERRRR DKQHLDDITAARLLPLHHMPTQLLS IEESLALQKQQKQNYEEMQAKLAAQ KLAERLNIKMRSYNPEGESS
  GRYREVRDEDDDWSSDEFTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Ion Channels: Other

Gene Information

• Gene Name: POLR2M RNA polymerase II subunit M [ Homo sapiens (human) ]
• Official Symbol: POLR2M
• Synonyms: GCOM1; Gdown; Gdown1; GRINL1A
• Gene ID: 81488
• mRNA Refseq: NM_015532.5
• Protein Refseq: NP_056347.1
• MIM: 606485
• UniProt ID: Q6EEV4