Active Recombinant Full Length Human PRKCA Protein, N-His-tagged

• Cat.No.: PRKCA-153HFL
• Product Overview: N-terminal 6Histagged recombinant human full length PKCα expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein kinase Ca (PKCa) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.203mg/ml of enzyme in 20mM Tris/HCl pH7.5, 5% glycerol, 10mM benzamidine, 1mM PMSF, 1mM EGTA, 1mM EDTA, 0.02% Triton X-100, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3597U/mg, where one unit of PKC alpha activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78 kDa
• AA Sequence: 1 MHHHHHHMAD VFPGNDSTAS QDVANRFARK GALRQKNVHE VKDHKFIARF FKQPTFCSHC 61 TDFIWGFGKQ GFQCQVCCFV VHKRCHEFVT FSCPGADKGP DTDDPRSKHK FKIHTYGSPT 121 FCDHCGSLLY GLIHQGMKCD TCDMNVHKQC VINVPSLCGM DHTEKRGRIY LKAEVADEKL 181 HVTVRDAKNL IPMDPNGLSD PYVKLKLIPD PKNESKQKTK TIRSTLNPQW NESFTFKLKP 241 SDKDRRLSVE IWDWDRTTRN DFMGSLSFGV SELMKMPASG WYKLLNQEEG EYYNVPIPEG 301 DEEGNMELRQ KFEKAKLGPA GNKVISPSED RKQPSNNLDR VKLTDFNFLM VLGKGSFGKV 361 MLADRKGTEE LYAIKILKKD VVIQDDDVEC TMVEKRVLAL LDKPPFLTQL HSCFQTVDRL 421 YFVMEYVNGG DLMYHIQQVG KFKEPQAVFY AAEISIGLFF LHKRGIIYRD LKLDNVMLDS 481 EGHIKIADFG MCKEHMMDGV TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWA YGVLLYEMLA 541 GQPPFDGEDE DELFQSIMEH NVSYPKSLSK EAVSICKGLM TKHPAKRLGC GPEGERDVRE 601 HAFFRRIDWE KLENREIQPP FKPKVCGKGA ENFDKFFTRG QPVLTPPDQL VIANIDQSDF 661 EGFSYVNPQF VHPILQSAV
• Purity: Purity 99% by SDS-PAGE and Coomassie Blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCA protein kinase C alpha [ Homo sapiens (human) ]
• Official Symbol: PRKCA
• Synonyms: PRKCA; protein kinase C alpha; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
• Gene ID: 5578
• mRNA Refseq: NM_002737.3
• Protein Refseq: NP_002728.2
• MIM: 176960
• UniProt ID: P17252