Active Recombinant Full Length Human PRKCQ Protein, C-His-tagged

• Cat.No.: PRKCQ-152HFL
• Product Overview: C-terminal 6Histagged, recombinant, full-length, human PKC theta, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Protein kinase C, theta (PKCtheta, PRKCQ, PRKCT) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.18mg/ml of enzyme in 50mM Hepes/NaOH pH7.5, 150mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 5% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 57U/mg, where one unit of PKC theta, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml Histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 82.8 kDa
• AA Sequence: 1 MSPFLRIGLS NFDCGSCQSC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 61 DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GKTEIWLELK PQGRMLMNAR 121 YFLEMSDTKD MNEFETEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 181 WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 241 SPTFCEHCGT LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ 301 ARCLRDTEQI FREGPVEIGL PCSIKNEARP PCLPTPGKRE PQGISWESPL DEVDKMCHLP 361 EPELNKERPS LQIKLKIEDF ILHKMLGKGS FGKVFLAEFK KTNQFFAIKA LKKDVVLMDD 421 DVECTMVEKR VLSLAWEHPF LTHMFCTFQT KENLFFVMEY LNGGDLMYHI QSCHKFDLSR 481 ATFYAAEIIL GLQFLHSKGI VYRDLKLDNI LLDKDGHIKI ADFGMCKENM LGDAKTNTFC 541 GTPDYIAPEI LLGQKYNHSV DWWSFGVLLY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR 601 WLEKEAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP FRPKVKSPFD 661 CSNFDKEFLN EKPRLSFADR ALINSMDQNM FRNFSFMNPG MERLISHHHH HH
• Purity: Purity 62% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCQ protein kinase C theta [ Homo sapiens (human) ]
• Official Symbol: PRKCQ
• Synonyms: PRKCQ; protein kinase C theta; PRKCT; nPKC-theta
• Gene ID: 5588
• mRNA Refseq: NM_006257.5
• Protein Refseq: NP_006248.1
• MIM: 600448
• UniProt ID: Q04759