Active Recombinant Full Length Human PRKD1 Protein, N-His-tagged

• Cat.No.: PRKD1-155HFL
• Product Overview: N-terminal 6His-tagged recombinant, full length, human PKC mu expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein kinase Cm (PKCm) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.222mg/ml of enzyme in 20mM Tris/HCl pH7.5, 0.02% Triton X-100, 5% glycerol, 1mM EGTA, 1mM EDTA, 10mM benzamidine, 1mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 414U/mg, where one unit of PKC mu, active activity is defined as 1nmol phosphate incorporated into 30μM (KKLNRTLSVA) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 105 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSMS APPVLRPPSP LLPVAAAAAA AAAALVPGSG 61 PGPAPFLAPV AAPVGGISFH LQIGLSREPV LLLQDSSGDY SLAHVREMAC SIVDQKFPEC 121 GFYGMYDKIL LFRHDPTSEN ILQLVKAASD IQEGDLIEVV LSASATFEDF QIRPHALFVH 181 SYRAPAFCDH CGEMLWGLVR QGLKCEGCGL NYHKRCAFKI PNNCSGVRRR RLSNVSLTGV 241 STIRTSSAEL STSAPDEPLL QKSPSESFIG REKRSNSQSY IGRPIHLDKI LMSKVKVPHT 301 FVIHSYTRPT VCQYCKKLLK GLFRQGLQCK DCRFNCHKRC APKVPNNCLG EVTINGDLLS 361 PGAESDVVME EGSDDNDSER NSGLMDDMEE AMVQDAEMAM AECQNDSGEM QDPDPDHEDA 421 NRTISPSTSN NIPLMRVVQS VKHTKRKSST VMKEGWMVHY TSKDTLRKRH YWRLDSKCIT 481 LFQNDTGSRY YKEIPLSEIL SLEPVKTSAL IPNGANPHCF EITTANVVYY VGENVVNPSS 541 PSPNNSVLTS GVGADVARMW EIAIQHALMP VIPKGSSVGT GTNLHRDISV SISVSNCQIQ 601 ENVDISTVYQ IFPDEVLGSG QFGIVYGGKH RKTGRDVAIK IIDKLRFPTK QESQLRNEVA 661 ILQNLHHPGV VNLECMFETP ERVFVVMEKL HGDMLEMILS SEKGRLPEHI TKFLITQILV 721 ALRHLHFKNI VHCDLKPENV LLASADPFPQ VKLCDFGFAR IIGEKSFRRS VVGTPAYLAP 781 EVLRNKGYNR SLDMWSVGVI IYVSLSGTFP FNEDEDIHDQ IQNAAFMYPP NPWKEISHEA 841 IDLINNLLQV KMRKRYSVDK TLSHPWLQDY QTWLDLRELE CKIGERYITH ESDDLRWEKY 901 AGEQRLQYPT HLINPSASHS DTPETEETEM KALGERVSIL
• Purity: Purity 78.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKD1 protein kinase D1 [ Homo sapiens (human) ]
• Official Symbol: PRKD1
• Synonyms: PRKD1; protein kinase D1; PKD; PKCM; CHDED; PRKCM; PKC-MU
• Gene ID: 5587
• mRNA Refseq: NM_002742.3
• Protein Refseq: NP_002733.2
• MIM: 605435
• UniProt ID: Q15139