Active Recombinant Full Length Human PTGIS Protein, C-Flag-tagged

• Cat.No.: PTGIS-717HFL
• Product Overview: Recombinant Full Length Human PTGIS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme substrate
• Molecular Mass: 56.9 kDa
• AA Sequence: MAWAALLGLLAALLLLLLLSRRRTR RPGEPPLDLGSIPWLGYALDFGKDA ASFLTRMKEKHGDIFTILVG GRYVTVLLDPHSYDAVVWEPRTRLD FHAYAIFLMERIFDVQLPHYSPSDE KARMKLTLLHRELQALTEAM YTNLHAVLLGDATEAGSGWHEMGLL DFSYSFLLRAGYLTLYGIEALPRTH ESQAQDRVHSADVFHTFRQL DRLLPKLARGSLSVGDKDHMCSVKS RLWKLLSPARLARRAHRSKWLESYL LHLEEMGVSEEMQARALVLQ LWATQGNMGPAAFWLLLFLLKNPEA LAAVRGELESILWQAEQPVSQTTTL PQKVLDSTPVLDSVLSESLR LTAAPFITREVVVDLAMPMADGREF NLRRGDRLLLFPFLSPQRDPEIYTD PEVFKYNRFLNPDGSEKKDF YKDGKRLKNYNMPWGAGHNHCLGRS YAVNSIKQFVFLVLVHLDLELINAD VEIPEFDLSRYGFGLMQPEH
  DVPVRYRIRPTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways

Gene Information

• Gene Name: PTGIS prostaglandin I2 synthase [ Homo sapiens (human) ]
• Official Symbol: PTGIS
• Synonyms: CYP8; PGIS; PTGI; CYP8A1
• Gene ID: 5740
• mRNA Refseq: NM_000961.4
• Protein Refseq: NP_000952.1
• MIM: 601699
• UniProt ID: Q16647