Active Recombinant Full Length Human PTK2B Protein, N-His-tagged

• Cat.No.: PTK2B-164HFL
• Product Overview: N-Terminal 6Histagged, recombinant, full-length, human Pyk2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Proline-rich tyrosine kinase 2 (PTK2B) (also called CAKb for cell adhesion kinase b, RAFTK for related adhesion focal tyrosine kinase, or CADTK for calcium-dependent tyrosine kinase) is a non-receptor tyrosine kinase structurally related to focal adhesion kinase (FAK). PTK2B interacts with Src family kinases and focal adhesion proteins such as p130Crk-associated substrate (p130Cas) and its homologue p105 human enhancer of filamentation 1 (HEF1). PTK2B is particularly enriched in pulmonary vascular endothelial cells and lung, a major organ site for tumor metastasis. Inhibitors of PTK2B may be useful in the treatment of lung cancer.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.00mg/ml of enzyme in Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 25U/mg, where one unit of Pyk2, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 117.2 kDa
• AA Sequence: 1 MHHHHHHEFM SGVSEPLSRV KLGTLRRPEG PAEPMVVVPV DVEKEDVRIL KVCFYSNSFN 61 PGKNFKLVKC TVQTEIREII TSILLSGRIG PNIRLAECYG LRLKHMKSDE IHWLHPQMTV 121 GEVQDKYECL HVEAEWRYDL QIRYLPEDFM ESLKEDRTTL LYFYQQLRND YMQRYASKVS 181 EGMALQLGCL ELRRFFKDMP HNALDKKSNF ELLEKEVGLD LFFPKQMQEN LKPKQFRKMI 241 QQTFQQYASL REEECVMKFF NTLAGFANID QETYRCELIQ GWNITVDLVI GPKGIRQLTS 301 QDAKPTCLAE FKQIRSIRCL PLEEGQAVLQ LGIEGAPQAL SIKTSSLAEA ENMADLIDGY 361 CRLQGEHQGS LIIHPRKDGE KRNSLPQIPM LNLEARRSHL SESCSIESDI YAEIPDETLR 421 RPGGPQYGIA REDVVLNRIL GEGFFGEVYE GVYTNHKGEK INVAVKTCKK DCTLDNKEKF 481 MSEAVIMKNL DHPHIVKLIG IIEEEPTWII MELYPYGELG HYLERNKNSL KVLTLVLYSL 541 QICKAMAYLE SINCVHRDIA VRNILVASPE CVKLGDFGLS RYIEDEDYYK ASVTRLPIKW 601 MSPESINFRR FTTASDVWMF AVCMWEILSF GKQPFFWLEN KDVIGVLEKG DRLPKPDLCP 661 PVLYTLMTRC WDYDPSDRPR FTELVCSLSD VYQMEKDIAM EQERNARYRT PKILEPTAFQ 721 EPPPKPSRPK YRPPPQTNLL APKLQFQVPE GLCASSPTLT SPMEYPSPVN SLHTPPLHRH 781 NVFKRHSMRE EDFIQPSSRE EAQQLWEAEK VKMRQILDKQ QKQMVEDYQW LRQEEKSLDP 841 MVYMNDKSPL TPEKEVGYLE FTGPPQKPPR LGAQSIQPTA NLDRTDDLVY LNVMELVRAV 901 LELKNELCQL PPEGYVVVVK NVGLTLRKLI GSVDDLLPSL PSSSRTEIEG TQKLLNKDLA 961 ELINKMRLAQ QNAVTSLSEE CKRQMLTASH TLAVDAKNLL DAVDQAKVLA NLAHPPAE
• Purity: Purity 59% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTK2B protein tyrosine kinase 2 beta [ Homo sapiens (human) ]
• Official Symbol: PTK2B
• Synonyms: PTK2B; protein tyrosine kinase 2 beta; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• Gene ID: 2185
• mRNA Refseq: NM_004103.4
• Protein Refseq: NP_004094.3
• MIM: 601212
• UniProt ID: Q14289