Active Recombinant Full Length Human RPS6KB1 Protein, C-Flag-tagged

• Cat.No.: RPS6KB1-233HFL
• Product Overview: Recombinant Full Length Human RPS6KB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA reaction
• Molecular Mass: 59 kDa
• AA Sequence: MRRRRRRDGFYPAPDFRDREAEDMA GVFDIDLDQPEDAGSEDELEEGGQL NESMDHGGVGPYELGMEHCE KFEISETSVNRGPEKIRPECFELLR VLGKGGYGKVFQVRKVTGANTGKIF AMKVLKKAMIVRNAKDTAHT KAERNILEEVKHPFIVDLIYAFQTG GKLYLILEYLSGGELFMQLEREGIF MEDTACFYLAEISMALGHLH QKGIIYRDLKPENIMLNHQGHVKLT DFGLCKESIHDGTVTHTFCGTIEYM APEILMRSGHNRAVDWWSLG ALMYDMLTGAPPFTGENRKKTIDKI LKCKLNLPPYLTQEARDLLKKLLKR NAASRLGAGPGDAGEVQAHP FFRHINWEELLARKVEPPFKPLLQS EEDVSQFDSKFTRQTPVDSPDDSTL SESANQVFLGFTYVAPSVLE SVKEKFSFEPKIRSPRRFIGSPRTP VSPVKFSPGDFWGRGASASTANPQT PVEYPMETSGIEQMDVTMSG
  EASAPLPIRQPNSGPYKKQAFPMIS KRPEHLRMNLTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase B1 [ Homo sapiens (human) ]
• Official Symbol: RPS6KB1
• Synonyms: S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• Gene ID: 6198
• mRNA Refseq: NM_003161.4
• Protein Refseq: NP_003152.1
• MIM: 608938
• UniProt ID: P23443

Reviews

09/16/2021

Dependable protein purification, consistently high-quality results.

06/11/2021

Precise mass spectrometry analysis, essential for our proteomics work.

06/30/2019

Efficient peptide synthesis, crucial for our peptide research projects.

Q&As

What are the mechanisms by which RPS6KB1 modulates autophagy and cell survival?

RPS6KB1 regulates autophagy and cell survival, crucial for cellular health and response to stress.

How does RPS6KB1 (Ribosomal Protein S6 Kinase B1) regulate protein synthesis and cell growth?

RPS6KB1 controls protein synthesis and cell growth, playing a key role in cellular proliferation.

What is the impact of RPS6KB1 on cellular responses to stress and DNA damage?

RPS6KB1 impacts cellular stress responses, including the repair of DNA damage and maintenance of genomic stability.

How does RPS6KB1 contribute to the development and progression of cancer?

RPS6KB1 can contribute to cancer by promoting tumor cell growth and proliferation.

What role does RPS6KB1 play in the mTOR signaling pathway, particularly in response to nutrient availability?

RPS6KB1 is a critical component of the mTOR pathway, responding to nutrient signals to regulate cell metabolism and growth.

How do alterations in RPS6KB1 expression or activity affect aging and age-related diseases?

Changes in RPS6KB1 function can affect aging processes and the development of age-related diseases.

How does RPS6KB1 influence metabolic processes, especially in conditions like obesity and diabetes?

RPS6KB1 influences metabolic processes, affecting how cells respond to conditions like obesity and diabetes.