Active Recombinant Full Length Human TLK1 Protein, N-His-tagged

• Cat.No.: TLK1-178HFL
• Product Overview: N-terminal 6Histagged, recombinant, human TLK1 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: TLK1 protein is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.823mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 293U/mg, where one unit of TLK1, active activity is defined as 1nmol phosphate incorporated into casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 91 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MSVQSSSGSL EGPPSWSQLS TSPTPGSAAA 61 ARSLLNHTPP SGRPREGAMD ELHSLDPRRQ ELLEARFTGV ASGSTGSTGS CSVGAKASTN 121 NESSNHSFGS LGSLSDKESE TPEKKQSESS RGRKRKAENQ NESSQGKSIG GRGHKISDYF 181 EYQGGNGSSP VRGIPPAIRS PQNSHSHSTP SSSVRPNSPS PTALAFGDHP IVQPKQLSFK 241 IIQTDLTMLK LAALESNKIQ DLEKKEGRID DLLRANCDLR RQIDEQQKLL EKYKERLNKC 301 ISMSKKLLIE KSTQEKLSSR EKSMQDRLRL GHFTTVRHGA SFTEQWTDGF AFQNLVKQQE 361 WVNQQREDIE RQRKLLAKRK PPTANNSQAP STNSEPKQRK NKAVNGAEND PFVRPNLPQL 421 LTLAEYHEQE EIFKLRLGHL KKEEAEIQAE LERLERVRNL HIRELKRINN EDNSQFKDHP 481 TLNERYLLLH LLGRGGFSEV YKAFDLYEQR YAAVKIHQLN KSWRDEKKEN YHKHACREYR 541 IHKELDHPRI VKLYDYFSLD TDTFCTVLEY CEGNDLDFYL KQHKLMSEKE ARSIVMQIVN 601 ALRYLNEIKP PIIHYDLKPG NILLVDGTAC GEIKITDFGL SKIMDDDSYG VDGMDLTSQG 661 AGTYWYLPPE CFVVGKEPPK ISNKVDVWSV GVIFFQCLYG RKPFGHNQSQ QDILQENTIL 721 KATEVQFPVK PVVSSEAKAF IRRCLAYRKE DRFDVHQLAN DPYLLPHMRR SNSSGNLHMA 781 GLTASPTPPS SSIITY
• Purity: Purity 89% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TLK1 tousled like kinase 1 [ Homo sapiens (human) ]
• Official Symbol: TLK1
• Synonyms: TLK1; tousled like kinase 1; PKU-beta
• Gene ID: 9874
• mRNA Refseq: NM_012290.5
• Protein Refseq: NP_036422.3
• MIM: 608438
• UniProt ID: Q9UKI8