Active Recombinant Full Length Human TSPO Protein, C-Flag-tagged

• Cat.No.: TSPO-142HFL
• Product Overview: Recombinant Full Length Human TSPO Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Surface Plasmon Ressonance (SPR)
• Molecular Mass: 18.6 kDa
• AA Sequence: MAPPWVPAMGFTLAPSLGCFVGSRF VHGEGLRWYAGLQKPSWHPPHWVLG PVWGTLYSAMGYGSYLVWKE LGGFTEKAVVPLGLYTGQLALNWAW PPIFFGARQMGWALVDLLLVSGAAA ATTVAWYQVSPLAARLLYPY
  LAWLAFATTLNYCVWRDNHGWHGGR RLPETRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction

Gene Information

• Gene Name: TSPO translocator protein [ Homo sapiens (human) ]
• Official Symbol: TSPO
• Synonyms: DBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18; TSPO1
• Gene ID: 706
• mRNA Refseq: NM_000714.6
• Protein Refseq: NP_000705.2
• MIM: 109610
• UniProt ID: P30536

Reviews

01/28/2023

Prolonged transportation will not affect the stability of the product.

07/11/2021

The price of this one is very reasonable, and the quality is very good, I am very satisfied.

02/13/2020

It has high solubility and can be dissolved quickly.

Q&As

How does TSPO affect neuroprotection?

TSPO has an impact on neuroprotection by modulating mitochondrial function to affect the survival and death of nerve cells. Specific mechanisms include inhibition of apoptosis and alleviation of oxidative stress.

What are the challenges and prospects of current research on TSPO?

At present, the research on TSPO faces some challenges, such as the difference in the expression of TSPO in various tissues, the functional changes of TSPO in different disease states, and the interaction between TSPO and other molecules. However, with the continuous advancement of technology and the in-depth understanding of the functions of TSPO, it is expected that there will be greater breakthroughs in drug design and treatment strategies for TSPO in the future.

How is TSPO involved in the synthesis and transport of cholesterol?

This protein regulates the permeability of mitochondrial membranes to cholesterol by binding to cholesterol transporters, thereby affecting cholesterol synthesis and trafficking.

Is TSPO associated with a variety of diseases,

Since TSPO is associated with a variety of diseases,it may be a therapeutic target for a variety of diseases, such as neurodegenerative diseases, cancer, and mental disorders. Some progress has been made in the design and development of drugs for TSPO.

How to detect the expression and function of TSPO?

Commonly used TSPO detection methods include immunohistochemistry, western blotting, flow cytometry, etc. In addition, TSPO ligands can also be used for binding experiments or functional experiments to study their biological functions.

What circumstances does the expression level of TSPO change?

The expression level of TSPO changes in a variety of physiological and pathological conditions, such as hypoxia, ischemia, inflammation, aging, etc. These changes affect the function of the mitochondria, which affects the survival and death of cells.