Active Recombinant Full Length Human TSSK4 Protein, N-GST,C-His-tagged

• Cat.No.: TSSK4-182HFL
• Product Overview: N-terminal GST tagged and Cterminal 6His tagged, recombinant, human TSSK4 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity and size exclusion chromatography.

Specification

• Description: TSSK4 (Testis-specific serine/threonine-protein kinase 4) is a serine/threonine protein kinase that is primarily expressed in the testis, where it plays a role in the regulation of spermatogenesis. TSSK4 has been shown to be involved in the regulation of sperm motility and morphology, and alterations in TSSK4 expression and activity have been linked to male infertility. Research has suggested that TSSK4 may also play a role in cancer. TSSK4 has been shown to be overexpressed in several types of cancer, including breast cancer, and high levels of TSSK4 expression have been associated with poor prognosis. Additionally, TSSK4 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: Full Length
• Form: 0.38mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 198U/mg, where one unit of TSSK4 activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 65 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MGKGDVLEAA 241 PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK ASDDYLNKFL 301 PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC SEPLAGKWFS 361 QLTLGIAYLH SKSIVHRDLK LENLLLDKWE NVKISDFGFA KMVPSNQPVG CSPSYRQVNC 421 FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD DTNLKKLLRE 481 TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ PTHEIRLLEA 541 MCQLHNTTKQ HQSLQITTHH HHHH
• Purity: Purity 46% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TSSK4 testis specific serine kinase 4 [ Homo sapiens (human) ]
• Official Symbol: TSSK4
• Synonyms: TSSK4; testis specific serine kinase 4; TSK4; TSK-4; TSSK5; STK22E; C14orf20
• Gene ID: 283629
• mRNA Refseq: NM_174944.4
• Protein Refseq: NP_777604.2
• MIM: 610711
• UniProt ID: Q6SA08