| Description : |
Src family kinases, Src, Fyn, and Yes, are expressed in numerous cell types, including neurons and muscle, and their activities are implicated in multiple signaling pathways. YES kinase is believed to play critical roles in tumor growth, angiogenesis, invasion, and dissemination. YES1 (YES) kinase has been implicated in several human cancers, such as advanced colon cancers and endometrial cancer. YES1 kinase inhibitors may be of therapeutic use in the treatment of human cancer. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
4.505mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
5558U/mg, where one unit of Yes activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu,Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
62.7 kDa |
| AA Sequence : |
1 MHHHHHHMGC IKSKENKSPA IKYRPENTPE PVSTSVSHYG AEPTTVSPCP SSSAKGTAVN 61 FSSLSMTPFG GSSGVTPFGG ASSSFSVVPS SYPAGLTGGV TIFVALYDYE ARTTEDLSFK 121 KGERFQIINN TEGDWWEARS IATGKNGYIP SNYVAPADSI QAEEWYFGKM GRKDAERLLL 181 NPGNQRGIFL VRESETTKGA YSLSIRDWDE IRGDNVKHYK IRKLDNGGYY ITTRAQFDTL 241 QKLVKHYTEH ADGLCHKLTT VCPTVKPQTQ GLAKDAWEIP RESLRLEVKL GQGCFGEVWM 301 GTWNGTTKVA IKTLKPGTMM PEAFLQEAQI MKKLRHDKLV PLYAVVSEEP IYIVTEFMSK 361 GSLLDFLKEG DGKYLKLPQL VDMAAQIADG MAYIERMNYI HRDLRAANIL VGENLVCKIA 421 DFGLARLIED NEYTARQGAK FPIKWTAPEA ALYGRFTIKS DVWSFGILQT ELVTKGRVPY 481 PGMVNREVLE QVERGYRMPC PQGCPESLHE LMNLCWKKDP DERPTFEYIQ SFLEDYFTAT 541 EPQYQPGENL |
| Purity : |
Purity 69.6% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
