Active Recombinant Full Length Mouse LYN Protein, N-His-tagged

• Cat.No.: LYN-96MFL
• Product Overview: N-terminal 6Histagged, recombinant full-length, mouse Lyn, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The Lyn protein is a member of the Src family of non-receptor-associated protein-tyrosine kinases. LYN A differs from LYN B in having an additional 21 amino acids at the amino terminus. Lyn undergoes a concentration-dependent autophosphorylation, thus activating the catalytic site and decreasing the accessibility of the SH2 domain. Lyn phosphorylates and activates Tec, non-receptor tyrosine kinase that is coexpressed with Lyn and acts downstream of Lyn in intracellular signaling pathways. Lyn tyrosine kinase has been shown to be necessary for IgE-receptor (FceRI)-mediated mast-cell activation.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: Full Length
• Form: 2.631mg/ml of enzyme in 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5% glycerol. Frozen solution.
• Bio-activity: 1610U/mg, where one unit of Lyn (mouse) activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 60 kDa
• AA Sequence: 1 MHHHHHHMGC IKSKRKDNLN DDEVDSKTQP VRNTDRTIYV RDPTSNKQQR PVPEFHLLPG 61 QRFQTKDPEE QGDIVVALYP YDGIHPDDLS FKKGEKMKVL EEHGEWWKAK SLSSKREGFI 121 PSNYVAKVNT LETEEWFFKD ITRKDAERQL LAPGNSAGAF LIRESETLKG SFSLSVRDYD 181 PMHGDVIKHY KIRSLDNGGY YISPRITFPC ISDMIKHYQK QSDGLCRRLE KACISPKPQK 241 PWDKDAWEIP RESIKLVKKL GAGQFGEVWM GYYNNSTKVA VKTLKPGTMS VQAFLEEANL 301 MKTLQHDKLV RLYAVVTKEE PIYIITEFMA KGSLLDFLKS DEGGKVLLPK LIDFSAQIAE 361 GMAYIERKNY IHRDLRAANV LVSESLMCKI ADFGLARVIE DNEYTAREGA KFPIKWTAPE 421 AINFGCFTIK SDVWSFGILL YEIVTYGKIP YPGRTNADVM SALSQGYRMP RMENCPDELY 481 DIMKMCWKEK AEERPTFDYL QSVLDDFYTA TEGQYQQQP
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Lyn LYN proto-oncogene, Src family tyrosine kinase [ Mus musculus (house mouse) ]
• Official Symbol: LYN
• Synonyms: LYN; LYN proto-oncogene, Src family tyrosine kinase; Hck-2; p53Lyn; p56Lyn
• Gene ID: 17096
• mRNA Refseq: NM_010747.3
• Protein Refseq: NP_034877.2
• UniProt ID: P25911