Active Recombinant Full Length Mouse MAPK1 Protein, N-GST-tagged

• Cat.No.: MAPK1-99MFL
• Product Overview: N-terminal GST-tagged, recombinant, full-length, mouse MAP Kinase 2, expressed in E.coli cells. Purified using glutathione agarose. Activated using MEK1 (cat# 14-429 and repurified using Ni2+/NTA agarose).

Specification

• Description: MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs.
• Source: E. coli
• Species: Mouse
• Tag: GST
• Protein length: Full Length
• Form: 0.7665mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol. Liquid at -20 centigrade.
• Bio-activity: 1900U/mg, where one unit of MAP Kinase 2, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.8 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSNMAA AAAAGPEMVR 241 GQVFDVGPRY TNLSYIGEGA YGMVCSAYDN LNKVRVAIKK ISPFEHQTYC QRTLREIKIL 301 LRFRHENIIG INDIIRAPTI EQMKDVYIVQ DLMETDLYKL LKTQHLSNDH ICYFLYQILR 361 GLKYIHSANV LHRDLKPSNL LLNTTCDLKI CDFGLARVAD PDHDHTGFLT EYVATRWYRA 421 PEIMLNSKGY TKSIDIWSVG CILAEMLSNR PIFPGKHYLD QLNHILGILG SPSQEDLNCI 481 INLKARNYLL SLPHKNKVPW NRLFPNADSK ALDLLDKMLT FNPHKRIEVE QALAHPYLEQ 541 YYDPSDEPIA EAPFKFDMEL DDLPKEKLKE LIFEETARFQ PGYRS
• Purity: Purity 96.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Mapk1 mitogen-activated protein kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: MAPK1
• Synonyms: MAPK1; mitogen-activated protein kinase 1; ERK; Erk2; MAPK2; PRKM2; Prkm1; p41mapk; p42mapk; 9030612K14Rik
• Gene ID: 26413
• mRNA Refseq: NM_011949.3
• Protein Refseq: NP_036079.1
• UniProt ID: P63085