Active Recombinant Human ABL2(38-end) Protein, N-His-tagged

• Cat.No.: ABL2-22H
• Product Overview: N-terminal 6Histagged recombinant human Arg residues 38–end. This is expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: The mammalian Abelson (Abl) family of non-receptor tyrosine kinases is comprised of c-Abl (ABL1) and Arg (Abl-related gene, ABL2), which are encoded by the Abl1 and Abl2 genes, respectively. Deregulation of the c-Abl and Arg tyrosine kinases can occur as a result of chromosomal translocation events that produce chimeric proteins associated with the development of human leukemias as well as gastric cancer invasion and progression.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 38-end
• Form: 0.775mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: 1948U/mg, where one unit of Arg, active activity is defined as 1nmol phosphate incorporated into 50μM Abltide (EAIYAAPFAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 121.9 kDa
• AA Sequence: 1 MHHHHHHEFE ALHRPYGCDV EPQALNEAIR WSSKENLLGA TESDPNLFVA LYDFVASGDN 61 TLSITKGEKL RVLGYNQNGE WSEVRSKNGQ GWVPSNYITP VNSLEKHSWY HGPVSRSAAE 121 YLLSSLINGS FLVRESESSP GQLSISLRYE GRVYHYRINT TADGKVYVTA ESRFSTLAEL 181 VHHHSTVADG LVTTLHYPAP KCNKPTVYGV SPIHDKWEME RTDITMKHKL GGGQYGEVYV 241 GVWKKYSLTV AVKTLKEDTM EVEEFLKEAA VMKEIKHPNL VQLLGVCTLE PPFYIVTEYM 301 PYGNLLDYLR ECNREEVTAV VLLYMATQIS SAMEYLEKKN FIHRDLAARN CLVGENHVVK 361 VADFGLSRLM TGDTYTAHAG AKFPIKWTAP ESLAYNTFSI KSDVWAFGVL LWEIATYGMS 421 PYPGIDLSQV YDLLEKGYRM EQPEGCPPKV YELMRACWKW SPADRPSFAE THQAFETMFH 481 DSSISEEVAE ELGRAASSSS VVPYLPRLPI LPSKTRTLKK QVENKENIEG AQDATENSAS 541 SLAPGFIRGA QASSGSPALP RKQRDKSPSS LLEDAKETCF TRDRKGGFFS SFMKKRNAPT 601 PPKRSSSFRE MENQPHKKYE LTGNFSSVAS LQHADGFSFT PAQQEANLVP PKCYGGSFAQ 661 RNLCNDDGGG GGGSGTAGGG WSGITGFFTP RLIKKTLGLR AGKPTASDDT SKPFPRSNST 721 SSMSSGLPEQ DRMAMTLPRN CQRSKLQLER TVSTSSQPEE NVDRANDMLP KKSEESAAPS 781 RERPKAKLLP RGATALPLRT PSGDLAITEK DPPGVGVAGV AAAPKGKEKN GGARLGMAGV 841 PEDGEQPGWP SPAKAAPVLP TTHNHKVPVL ISPTLKHTPA DVQLIGTDSQ GNKFKLLSEH 901 QVTSSGDKDR PRRVKPKCAP PPPPVMRLLQ HPSICSDPTE EPTALTAGQS TSETQEGGKK 961 AALGAVPISG KAGRPVMPPP QVPLPTSSIS PAKMANGTAG TKVALRKTKQ AAEKISADKI 1021 SKEALLECAD LLSSALTEPV PNSQLVDTGH QLLDYCSGYV DCIPQTRNKF AFREAVSKLE 1081 LSLQELQVSS AAAGVPGTNP VLNNLLSCVQ EISDVVQR
• Purity: Purity 32.7% by SDSPAGE and Coomassie staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ABL2 ABL proto-oncogene 2, non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ABL2
• Synonyms: ABL2; ABL proto-oncogene 2, non-receptor tyrosine kinase; ARG; ABLL
• Gene ID: 27
• mRNA Refseq: NM_005158.5
• Protein Refseq: NP_005149.4
• MIM: 164690
• UniProt ID: P42684