Active Recombinant Human ACAT1 Protein, His-tagged

• Cat.No.: ACAT1-01H
• Product Overview: E. coli-derived human ACAT1 protein
  Val34-Leu427 with an N-terminal Met and 6-His tag.

Specification

• Description: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
• Source: Escherichia coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to convert acetoacetyl-CoA and CoA into acetyl-CoA. The specific activity is >15000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 42 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 °C as supplied.
  3 months, -20 to -70 °C under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and TCEP.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: ACAT1 acetyl-CoA acetyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: ACAT1
• Synonyms: ACAT; ACAT1; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; EC 2.3.1; EC 2.3.1.9; MAT; mitochondrial acetoacetyl-CoA thiolase; T2; THIL
• Gene ID: 38
• mRNA Refseq: NM_000019.4
• Protein Refseq: NP_000010.1
• MIM: 607809
• UniProt ID: P24752

Reviews

01/16/2023

The recombinant human ACAT1 protein was affordable and highly effective.

09/16/2022

The customization services provided by Creative BioMart's technical support team for the recombinant ACAT1 protein were invaluable. I am grateful for their expertise and professionalism.

10/07/2020

I appreciate the level of expertise and professionalism demonstrated by Creative BioMart's scientists. Their Recombinant Human ACAT1 Protein helped me achieve the results I needed for my project.

Q&As

I want to buy ACAT1 protein, is there any discount?

Creative BioMart provides coupons for each new customer and runs promotions on holidays. For specific promotions, please contact our sales staff for more information.

What is the role of ACAT1 in NAFLD?

Studies have demonstrated that upregulation of ACAT1 expression and activity can lead to the development of hepatic steatosis, a hallmark of NAFLD. Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD.

Can I get a discount on the price of recombinant ACAT1 protein?

Coupons are available for every new customer, and we run promotions on important holidays. For specific promotions, please contact our sales staff for more information.

What is the significance of ACAT1 in lipid metabolism?

ACAT1 is a key enzyme involved in the biosynthesis of cholesterol and ketone bodies, as well as in the breakdown of fatty acids through beta-oxidation. It plays a critical role in maintaining lipid homeostasis in the liver and regulating the levels of circulating cholesterol and fatty acids.

How is ACAT1 regulated in the liver?

ACAT1 expression is regulated by a variety of factors, including hormonal signals such as insulin and glucagon, as well as by the levels of intracellular metabolites such as acetyl-CoA and cholesterol. In addition, ACAT1 activity is subject to post-translational modifications such as phosphorylation, which can modulate its enzymatic activity.

What is the structural basis for ACAT1 function?

ACAT1 is a homotetrameric protein that consists of four identical subunits. Each subunit contains a catalytic domain that is responsible for the transfer of acetyl-CoA, as well as a regulatory domain that modulates enzymatic activity. The crystal structure of ACAT1 has been solved, revealing key structural features such as the location of the active site and the binding sites for acetyl-CoA and cholesterol.

Can recombinant ACAT1 protein be used as a diagnostic or therapeutic agent?

Our recombinant ACAT1 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes.

What are the potential therapeutic applications of ACAT1 inhibition?

Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD, as well as other metabolic disorders such as hypercholesterolemia and atherosclerosis.

How can the recombinant ACAT1 protein be used in research or applications?

The recombinant ACAT1 protein can be used in a variety of research and application settings, such as studying the biochemistry and enzymology of the ACAT1 enzyme, screening for inhibitors or activators of the enzyme, or developing diagnostic assays for diseases related to cholesterol metabolism. The protein can also be used as a reference standard or control in analytical methods such as mass spectrometry or ELISA. However, it is important to note that the specific use of the recombinant ACAT1 protein will depend on the goals and requirements of the individual research or application.

How is the recombinant ACAT1 protein quantified and characterized?

Recombinant protein is typically quantified using a combination of techniques, such as UV spectrophotometry, Bradford protein assay, or bicinchoninic acid (BCA) assay. To characterize the protein, a variety of methods can be used, including SDS-PAGE, Western blotting, mass spectrometry, and enzyme activity assays.