Active Recombinant human ALT2/GPT2 Protein, His-tagged

• Cat.No.: GPT2-01H
• Product Overview: Recombinant human GPT2 protein (1-523aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His-Tag
• Form: Liquid
• Bio-activity: Specific activity is > 100 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37 centigrade.
• Molecular Mass: 60.3 kDa (546aa)
• Protein length: 1-523aa
• AA Sequence: MQRAAALVRRGCGPRTPSSWGRSQS SAAAEASAVLKVRPERSRRERILTL ESMNPQVKAVEYAVRGPIVLKAGEI ELELQRGIKKPFTEVIRANIGDAQA MGQQPITFLRQVMALCTYPNLLDSP SFPEDAKKRARRILQACGGNSLGSY SASQGVNCIREDVAAYITRRDGGVP ADPDNIYLTTGASDGISTILKILVS GGGKSRTGVMIPIPQYPLYSAVISE LDAIQVNYYLDEENCWALNVNELRR AVQEAKDHCDPKVLCIINPGNPTGQ VQSRKCIEDVIHFAWEEKLFLLADE VYQDNVYSPDCRFHSFKKVLYEMGP EYSSNVELASFHSTSKGYMGECGYR GGYMEVINLHPEIKGQLVKLLSVRL CPPVSGQAAMDIVVNPPVAGEESFE QFSREKESVLGNLAKKAKLTEDLFN QVPGIHCNPLQGAMYAFPRIFIPAK AVEAAQAHQMAPDMFYCMKLLEETG ICVVPGSGFGQREGTYHFRMTILPP VEKLKTVLQKVKDFHINFLEKYA
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 2 mM DTT, 0.2 M NaCl

Gene Information

• Gene Name: GPT2 glutamic--pyruvic transaminase 2 [ Homo sapiens (human) ]
• Official Symbol: GPT2
• Synonyms: GPT2; glutamic--pyruvic transaminase 2; ALT2; GPT 2; MRT49; NEDSPM; alanine aminotransferase 2; glutamate pyruvate transaminase 2; glutamic pyruvate transaminase (alanine aminotransferase) 2; glutamic pyruvate transaminase 2; glutamic--alanine transaminase 2; EC 2.6.1.2
• Gene ID: 84706
• mRNA Refseq: NM_133443
• Protein Refseq: NP_597700
• MIM: 138210
• UniProt ID: Q8TD30