Active Recombinant Human ATG7, His-tagged
Cat.No. : | ATG7-169H |
Product Overview : | Recombinant human ATG7 (amino acid residues 1-706), fused with N-terminal His, was expressed in Insect sf21. |
- Specification
- Gene Information
- Related Products
Description : | Ubiquitin-like proteins (ubl) Autophagy 12 (ATG12) and ATG8 are activated by ATG7 the E1-like activating enzyme. ATG12 and ATG8 are transferred to two E2-like conjugating enzymes ATG10 and ATG3 respectively. Cloning of the human ATG7 gene was first described by Yuan et al. (1999) and ATG7 shares 38% sequence identity to its yeast homologue Apg7. ATG7 can also activate ATG8 mammalian homologues, GABARAP and GATE-16. It forms a homodimer via the C-terminal region that is important for enzyme substrate interaction and E1-E2 complex formation (Komatsu et al. 2005). |
Source : | Insect cells |
Species : | Human |
Tag : | His |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E1 Thioester GABARAP Loading Assay: The activity of His-ATG7 was validated by loading GABARAP onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of GABARAP and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the GABARAP/His-ATG7 thioester bond to the reducing agent DTT was confirmed. |
Molecular Mass : | 81.34 kDa |
Purity : | >95% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) [ Homo sapiens ] |
Official Symbol : | ATG7 |
Synonyms : | ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); APG7 autophagy 7 like (S. cerevisiae) , APG7L; ubiquitin-like modifier-activating enzyme ATG7; DKFZp434N0735; GSA7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; APG7L; APG7-LIKE; |
Gene ID : | 10533 |
mRNA Refseq : | NM_001136031 |
Protein Refseq : | NP_001129503 |
MIM : | 608760 |
UniProt ID : | O95352 |
Chromosome Location : | 3p25.3-p25.2 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of autophagy, organism-specific biosystem; |
Function : | APG12 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein homodimerization activity; ubiquitin activating enzyme activity; |
Products Types
◆ Recombinant Protein | ||
Atg7-1762M | Recombinant Mouse Atg7 Protein, Myc/DDK-tagged | +Inquiry |
Atg7-1524M | Recombinant Mouse Atg7 Protein, His-tagged | +Inquiry |
ATG7-261H | Recombinant Human ATG7 Protein, His-tagged | +Inquiry |
ATG7-501R | Recombinant Rat ATG7 Protein, His (Fc)-Avi-tagged | +Inquiry |
ATG7-272R | Recombinant Rhesus Macaque ATG7 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
ATG7-8621HCL | Recombinant Human ATG7 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket