Active Recombinant Human AZGP1, His-tagged

• Cat.No.: AZGP1-296H
• Product Overview: Recombinant Human AZGP1 (Accession # AAH05306) Met1-Ser298, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MC3T3-E1 mouse preosteoblast cells. Ogikubo, O. et al. (1998) Biochem. Biophys. Res. Commun. 252(1):257. The ED50 for this effect is typically 0.1 - 0.6 µg/mL.
• Molecular Mass: Recombinant Human AZGP1, His-tagged has a calculated MW of 32.9 kDa. In SDS-PAGE migrates as 41-45 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: AZGP1 alpha-2-glycoprotein 1, zinc-binding [ Homo sapiens ]
• Official Symbol: AZGP1
• Synonyms: AZGP1; alpha-2-glycoprotein 1, zinc-binding; alpha 2 glycoprotein 1, zinc; zinc-alpha-2-glycoprotein; ZA2G; ZAG; zn-alpha-2-GP; Zn-alpha2-glycoprotein; zn-alpha-2-glycoprotein; Alpha-2-glycoprotein, zinc
• Gene ID: 563
• mRNA Refseq: NM_001185
• Protein Refseq: NP_001176
• MIM: 194460
• UniProt ID: P25311
• Chromosome Location: 7q22.1