Active Recombinant Human BDNF Protein

• Cat.No.: BDNF-144H
• Product Overview: Recombinant Human Pro-Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala19-Arg247(R125A,R127A,R128A) is expressed.

Specification

• Description: The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Bio-activity: Immobilized Human pro-BDNF at 2ug/ml (100 μl/well) can bind Human TrkB-His. The ED50 of Human pro-BDNF is 0.5-4 ug/ml.
• Molecular Mass: Predicted Molecular Weight: 25.6kDa
  Apparent Molecular Weight: 28kDa, reducing conditions.
• AA Sequence: Ala19-Arg247
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: BDNF brain derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: BDNF
• Synonyms: Brain-Derived Neurotrophic Factor; Abrineurin; ANON2; BULN2
• Gene ID: 627
• mRNA Refseq: NM_001143805.1
• Protein Refseq: NP_001137277.1
• MIM: 113505
• UniProt ID: P23560