| Description : |
Bone morphogenetic protein 4 (BMP-4) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-4 is widely expressed from early embryogenesis through dulthood. It plays an important role in cartilage and bone formation, mesoderm induction, tooth development, limb formation and fracture repair. Mature human BMP-4 is a 116 amino acids glycoprotein and normally found as a homodimer. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans. |
| Source : |
Human cells |
| Species : |
Human |
| Bio-activity : |
The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line). |
| Molecular Mass : |
34-40 kDa |
| Endotoxin : |
<0.5 EU/ μg |
| Purity : |
High Purity, >95% by SDS-PAGE |
| Stability : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein |
| Reconstitution : |
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
