Active Recombinant Human BMPR2 Protein, N-His-tagged

• Cat.No.: BMPR2-29H
• Product Overview: N-terminal 6His-tagged, recombinant fragment of human BMPR2 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: Bone Morphogenetic Protein Receptor Type 2 (BMPR2) is a member of the bone morphogenetic protein receptor family of transmembrane serine/threonine kinases. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.
• Source: Sf21
• Species: Human
• Tag: His
• Form: 1.85 mg/ml of enzyme in 40 mM Tris/HCl pH 7.5, 40 mM NaCl, 240 mM imidazole, 0.08 mM EGTA, 20% (v/v) glycerol. Frozen solution.
• Bio-activity: 8 U/mg, where one unit of BMPR2 activity is defined as 1 nmol phosphate incorporated into 0.33 mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 67 kDa
• Purity: 83% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BMPR2 bone morphogenetic protein receptor type 2 [ Homo sapiens (human) ]
• Official Symbol: BMPR2
• Synonyms: BMPR2; bone morphogenetic protein receptor type 2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• Gene ID: 659
• mRNA Refseq: NM_001204.7
• Protein Refseq: NP_001195.2
• MIM: 600799
• UniProt ID: Q13873