| Description : |
BrSK2 is a serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
2-end |
| Form : |
1.77mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
197U/mg, where one unit of BrSK2 activity is defined as 1nmol phosphate incorporated into 500μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
78.5 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMGSTS TGKDGGAQHA QYVGPYRLEK TLGKGQTGLV 61 KLGVHCVTCQ KVAIKIVNRE KLSESVLMKV EREIAILKLI EHPHVLKLHD VYENKKYLYL 121 VLEHVSGGEL FDYLVKKGRL TPKEARKFFR QIISALDFCH SHSICHRDLK PENLLLDEKN 181 NIRIADFGMA SLQVGDSLLE TSCGSPHYAC PEVIRGEKYD GRKADVWSCG VILFALLVGA 241 LPFDDDNLRQ LLEKVKRGVF HMPHFIPPDC QSLLRGMIEV DAARRLTLEH IQKHIWYIGG 301 KNEPEPEQPI PRKVQIRSLP SLEDIDPDVL DSMHSLGCFR DRNKLLQDLL SEEENQEKMI 361 YFLLLDRKER YPSQEDEDLP PRNEIDPPRK RVDSPMLNRH GKRRPERKSM EVLSVTDGGS 421 PVPARRAIEM AQHGQRSRSI SGASSGLSTS PLSSPRVTPH PSPRGSPLPT PKGTPVHTPK 481 ESPAGTPNPT PPSSPSVGGV PWRARLNSIK NSFLGSPRFH RRKLQVPTPE EMSNLTPESS 541 PELAKKSWFG NFISLEKEEQ IFVVIKDKPL SSIKADIVHA FLSIPSLSHS VISQTSFRAE 601 YKATGGPAVF QKPVKFQVDI TYTEGGEAQK ENGIYSVTFT LLSGPSRRFK RVVETIQAQL 661 LSTHDPPAAQ HLSEPPPPAP GLSWGAGLKG QKVATSYESS L |
| Purity : |
Purity 67% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
