Active Recombinant Human CAPZA1/CAPZB Protein, His-tagged
Cat.No. : | CAPZA1&CAPZB-869H |
Product Overview : | Recombinant Human CAPZA1&CAPZB(non-muscle isoform) with the alpha-1 SU untagged and beta-2 SU His-tagged was produced in HEK293 cells. |
- Specification
- Gene Information
- Related Products
Description : | The subunits of the heterodimeric capping protein CapZ possess a molecular mass of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with high affinity in a Ca++-independent manner to the barbed end of actin filaments (1:1000 M/M). Ca++-insensitive barbed end capping protein. Kd actin binding = >1nM (barbed end) . |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution. |
Bio-activity : | Capping activity: F-actin containing 20% pyrene actin was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ. Complete capping was obtained at a molar ratio of 1 : 1000 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4 centigrade. |
Molecular Mass : | 66kDa |
Purity : | >94% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Storage : | CapZ is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
Shipping : | At ambient temperature. Upon delivery store at -70 centigrade. |
Gene Name : | CAPZA1 capping protein (actin filament) muscle Z-line, alpha 1 [ Homo sapiens ] |
Official Symbol : | CAPZA1 |
Synonyms : | CAPZA1; capping protein (actin filament) muscle Z-line, alpha 1; F-actin-capping protein subunit alpha-1; Cap Z; capZ alpha-1; CAPZ; CAZ1; CAPPA1; |
Gene ID : | 829 |
mRNA Refseq : | NM_006135 |
Protein Refseq : | NP_006126 |
MIM : | 601580 |
UniProt ID : | P52907 |
Products Types
◆ Recombinant Protein | ||
CAPZA1-792R | Recombinant Rat CAPZA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Capza1-762M | Recombinant Mouse Capza1 Protein, MYC/DDK-tagged | +Inquiry |
CAPZA1-499H | Recombinant Human CAPZA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
CAPZA1-0382H | Recombinant Human CAPZA1 Protein, GST-Tagged | +Inquiry |
CAPZA1-454R | Recombinant Rhesus Macaque CAPZA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CAPZA1-7853HCL | Recombinant Human CAPZA1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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