Source : |
HEK293 |
Species : |
Human |
Tag : |
mFc |
Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant. |
Bio-activity : |
Immobilized Human CD19 (20-291), Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse / Human chimeric FMC63 MAb (human IgG1) with a linear range of 0.5-8 ng/mL. |
Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 57.0 kDa. The protein migrates as 75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Pro 20-Lys 291 |
Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |