Active Recombinant Human CD27 Protein, hIgG/His-tagged

• Cat.No.: CD27-06H
• Product Overview: Recombinant human CD27 (20-191aa), fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
• Source: HEK293
• Species: Human
• Tag: hIgG/His
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7. The ED50 range ≤ 0.8 μg/mL.
• Molecular Mass: 46.2 kDa (411aa)
• Protein length: 20-191aa
• AA Sequence: ATPAPKSCPERHYWAQGKLCCQMCE PGTFLVKDCDQHRKAAQCDPCIPGV SFSPDHHTRPHCESCRHCNSGLLVR NCTITANAECACRNGWQCRDKECTE CDPLPNPSLTARSSQALSPHPQPTH LPYVSEMLEARTAGHMQTLADFRQL PARTLSTHWPPQRSLCSSDFIR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens (human) ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; T14; S152; Tp55; TNFRSF7; S152. LPFS2; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233.1
• MIM: 186711
• UniProt ID: P26842