Active Recombinant Human CD40LG Protein, Fc/Avi-tagged, Biotinylated, Alexa Fluor 555 conjugated
Cat.No. : | CD40LG-280HAF555 |
Product Overview : | Biotinylated, Alexa Fluor 555 conjugated Human CD40 Ligand, Fc Tag is expressed from HEK293 cells. It contains AA Met 113 - Leu 261 (Accession # P29965-1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc/Avi |
Form : | Lyophilized |
Bio-activity : | Immobilized Human CD40, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand, Fc Tag with a linear range of 1-31 μg/mL. |
Molecular Mass : | The protein has a calculated MW of 45.0 kDa. The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
N-terminal Sequence Analysis : | Gly |
Endotoxin : | < 1.0 EU/ μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Stability : | Samples are stable for up to 12 months from date of receipt at -70 centigrade. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CD40LG CD40 ligand [ Homo sapiens ] |
Official Symbol : | CD40LG |
Synonyms : | CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5; |
Gene ID : | 959 |
mRNA Refseq : | NM_000074 |
Protein Refseq : | NP_000065 |
MIM : | 300386 |
UniProt ID : | P29965 |
Products Types
◆ Recombinant Protein | ||
CD40LG-12H | Active Recombinant Human CD40LG Protein (116-261), N-FLAG tagged, Certified Serum Grade | +Inquiry |
CD40LG-197H | Recombinant Human CD40LG Protein, C-His-tagged | +Inquiry |
CD40LG-634H | Recombinant Human CD40LG Protein | +Inquiry |
CD40LG-38H | Active Recombinant Human CD40LG Protein | +Inquiry |
CD40LG-680R | Recombinant Rhesus monkey CD40LG Protein, His-tagged | +Inquiry |
◆ Lysates | ||
CD40LG-1548MCL | Recombinant Mouse CD40LG cell lysate | +Inquiry |
CD40LG-001CCL | Recombinant Canine CD40LG cell lysate | +Inquiry |
CD40LG-1205RCL | Recombinant Rat CD40LG cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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