Active Recombinant Human CD80 protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | CD80-051H |
Product Overview : | Biotinylated Recombinant Human CD80(Val35-Asn242) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
- Related Products
Description : | B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30-kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). |
Source : | CHO cells |
Species : | Human |
Tag : | Fc/Avi |
Predicted N Terminal : | Val35 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human CTLA-4 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD80 Fc/Avi-tag that produces 50% of the optimal binding response is 0.4-2.4 ng/mL. |
Molecular Mass : | 77-89 kDa, under reducing conditions |
Protein length : | Val35-Asn242 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | CD80 CD80 molecule [ Homo sapiens ] |
Official Symbol : | CD80 |
Synonyms : | CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1; |
Gene ID : | 941 |
mRNA Refseq : | NM_005191 |
Protein Refseq : | NP_005182 |
MIM : | 112203 |
UniProt ID : | P33681 |
Products Types
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Cd80-37M | Recombinant Mouse CD80 Protein (ECD), His-tagged(C-ter) | +Inquiry |
Cd80-848M | Recombinant Mouse Cd80 Protein, MYC/DDK-tagged | +Inquiry |
Cd80-5171M | Active Recombinant Mouse Cd80 protein(Met1-Lys245), His-tagged | +Inquiry |
Cd80-24R | Recombinant Rat Cd80 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CD80-1767MCL | Recombinant Mouse CD80 cell lysate | +Inquiry |
CD80-948CCL | Recombinant Cynomolgus CD80 cell lysate | +Inquiry |
CD80-2715HCL | Recombinant Human CD80 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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