Active Recombinant Human CD8A&CD8B protein, His/FLAG-tagged
Cat.No. : | CD8A&CD8B-211H |
Product Overview : | Recombinant Human CD8A(Ser22-Asp182) fused with His tag at C-terminal and CD8B(Asn19-Pro170) fused with FLAG tag at N-terminal was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Description : | CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes . CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL), intraepithelial lymphocytes (IEL), some gamma δ T cells, subsets of thymic and splenic dendritic cells (DC), and peritoneal mast cells. It can form disulfide linked homodimers or heterodimers with CD8 beta. Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers. CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses. The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain. Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively. |
Source : | HEK293 |
Species : | Human |
Tag : | His/FLAG |
Predicted N Terminal : | Ser22 (CD8 alpha ) & Asn19 (CD8 beta ) |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Bio-activity : | Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D. et al. (2001) J. Immunol. 167:3099. The ED50 for this effect is 0.8-4 μg/mL. |
Molecular Mass : | Predicted Molecular Mass: 26 (CD8 alpha ) & 26 (CD8 beta ) kDa SDS-PAGE: 32-43 kDa, reducing conditions |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | CD8A CD8a molecule [ Homo sapiens ] |
Official Symbol : | CD8A |
Synonyms : | CD8; MAL; p32; Leu2 |
Gene ID : | 925 |
mRNA Refseq : | NM_001145873 |
Protein Refseq : | NP_001139345 |
MIM : | 186910 |
UniProt ID : | P01732 |
Products Types
◆ Recombinant Protein | ||
Cd8a-705M | Recombinant Mouse Cd8a Protein, His-tagged | +Inquiry |
CD8A-176H | Recombinant Human CD8A Protein, C-His-tagged | +Inquiry |
CD8A-922R | Recombinant Rat CD8A Protein, His (Fc)-Avi-tagged | +Inquiry |
CD8A-401B | Recombinant Bovine CD8A Protein (26-189 aa), His-SUMO-tagged | +Inquiry |
CD8A-0878H | Recombinant Human CD8A Protein, GST-Tagged | +Inquiry |
◆ Lysates | ||
CD8A-2493HCL | Recombinant Human CD8A cell lysate | +Inquiry |
CD8A-2514MCL | Recombinant Mouse CD8A cell lysate | +Inquiry |
CD8A-1872FCL | Recombinant Ferret CD8A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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