Active Recombinant Human CDC42BPB(1-473) Protein, N-His-tagged

• Cat.No.: CDC42BPB-158H
• Product Overview: N-terminal 6Histagged, recombinant, human MRCK amino acids 1–473, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: MRCKβ, also called CDC42 Binding Protein Kinase Beta, is a member of the serine/threonine protein kinase family. The protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-473 aa
• Form: 1.617mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 222U/mg, where one unit of MRCK activity is defined as 1nmol phosphate incorporated into 100μM (KKRNRTLTV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 55.5 kDa
• AA Sequence: 1 MSAKVRLKKL EQLLLDGPWR NESALSVETL LDVLVCLYTE CSHSALRRDK YVAEFLEWAK 61 PFTQLVKEMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER IYAMKILNKW EMLKRAETAC 121 FREERDVLVN GDCQWITALH YAFQDENHLY LVMDYYVGGD LLTLLSKFED KLPEDMARFY 181 IGEMVLAIDS IHQLHYVHRD IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP 241 DYISPEILQA MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF 301 QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI RNLEAPYIPD 361 VSSPSDTSNF DVDDDVLRNT EILPPGSHTG FSGLHLPFIG FTFTTESCFS DRGSLKSIMQ 421 SNTLTKDEDV QRDLEHSLQM EAYERRIRRL EQEKLELSRK LQESTQTVQS LHGHHHHHH
• Purity: Purity 71.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDC42BPB CDC42 binding protein kinase beta [ Homo sapiens (human) ]
• Official Symbol: CDC42BPB
• Synonyms: CDC42BPB; CDC42 binding protein kinase beta; MRCKB; CHOCNS
• Gene ID: 9578
• mRNA Refseq: NM_006035.4
• Protein Refseq: NP_006026.3
• MIM: 614062
• UniProt ID: Q9Y5S2