Active Recombinant Human CIRBP protein, His-tagged
Cat.No. : | CIRBP-27194TH |
Product Overview : | Recombinant Human CIRBP protein(NP_001271.1)(Met1~Glu172), fused to N-terminal His Tag, was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner. |
Molecular Mass : | 22/27kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Met1~Glu172 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | > 95% |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80ºC for 12 months. |
Concentration : | 400µg/mL |
Reconstitution : | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | CIRBP cold inducible RNA binding protein [ Homo sapiens ] |
Official Symbol : | CIRBP |
Synonyms : | CIRP; A18HNRNP |
Gene ID : | 1153 |
mRNA Refseq : | NM_001280.2 |
Protein Refseq : | NP_001271.1 |
MIM : | 602649 |
UniProt ID : | Q14011 |
Products Types
◆ Recombinant Protein | ||
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Cirbp-1779M | Recombinant Mouse Cirbp protein, His-tagged | +Inquiry |
◆ Lysates | ||
CIRBP-7491HCL | Recombinant Human CIRBP 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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