Active Recombinant Human CSNK1G3(25-355) Protein, N-His-tagged

• Cat.No.: CSNK1G3-42H
• Product Overview: N-terminal 6Histagged, recombinant, human Casein Kinase 1γ3, amino acids 1–330, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyper-phosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 25-355 aa
• Form: 3.81mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 20408U/mg, where one unit of Casein Kinase 1γ3, active activity is defined as 1nmol phosphate incorporated into 200μM (KRRRALS(p)VASLPGL) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 42.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MENKKKDKDK SDDRMARPSG RSGHNTRGTG 61 SSSSGVLMVG PNFRVGKKIG CGNFGELRLG KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY 121 EQLGSGDGIP QVYYFGPCGK YNAMVLELLG PSLEDLFDLC DRTFSLKTVL MIAIQLISRM 181 EYVHSKNLIY RDVKPENFLI GRPGNKTQQV IHIIDFGLAK EYIDPETKKH IPYREHKSLT 241 GTARYMSINT HLGKEQSRRD DLEALGHMFM YFLRGSLPWQ GLKADTLKER YQKIGDTKRA 301 TPIEVLCENF PEMATYLRYV RRLDFFEKPD YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL
• Purity: Purity 97% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CSNK1G3 casein kinase 1 gamma 3 [ Homo sapiens (human) ]
• Official Symbol: CSNK1G3
• Synonyms: CSNK1G3; casein kinase 1 gamma 3; CSNK1G3L; CKI-gamma 3
• Gene ID: 1456
• mRNA Refseq: NM_001031812.4
• Protein Refseq: NP_001026982.1
• MIM: 604253
• UniProt ID: Q9Y6M4