Active Recombinant Human CXCR4 protein

• Cat.No.: CXCR4-3046H
• Product Overview: Recombinant Human CXCR4 protein(Met 1-Ser 352) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human CXCR4 Full Length Protein-VLP at 5 μg/mL (100 μL/well) can bind Human ulocuplumab, Human IgG4 with a linear range of 1-31 ng/mL.
• Molecular Mass: Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening. The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
• Protein Length: Met 1-Ser 352
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CXCR4 chemokine (C-X-C motif) receptor 4 [ Homo sapiens ]
• Official Symbol: CXCR4
• Synonyms: CXCR4; chemokine (C-X-C motif) receptor 4; chemokine (C X C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CD184; D2S201E; fusin; HM89; HSY3RR; LESTR; NPY3R; NPYR; NPYY3R; CXC-R4; CXCR-4; CD184 antigen; SDF-1 receptor; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; FB22; LAP3; LCR1; WHIM; NPYRL
• Gene ID: 7852
• mRNA Refseq: NM_001008540
• Protein Refseq: NP_001008540
• MIM: 162643
• UniProt ID: P61073

Reviews

04/07/2022

The responsive customer support team provided quick resolutions to any queries or issues we encountered.

10/28/2021

The product's cost-effectiveness and clear instructions were instrumental in our experimental design.

10/29/2018

We found the product's straightforward protocols highly conducive to seamless integration into our lab work.

Q&As

How does CXCR4 participate in chemotaxis and migration of immune cells, particularly in the context of the immune response?

CXCR4 participates in chemotaxis and migration of immune cells, facilitating their movement toward specific tissues during the immune response.

Are there specific chemokines or ligands that CXCR4 binds to, influencing its role in immune cell activation and recruitment?

Specific chemokines or ligands, such as CXCL12, bind to CXCR4, influencing its role in immune cell activation and recruitment.

In which cellular compartments is CXCR4 predominantly localized, especially within immune cells?

CXCR4 is predominantly localized on the cell surface of immune cells, particularly within the membrane of T cells and monocytes.

What diseases or conditions are associated with dysregulation or abnormal expression of CXCR4, especially in the immune system?

Dysregulation or abnormal expression of CXCR4 is associated with diseases such as cancer, HIV/AIDS, and autoimmune disorders, particularly affecting immune cell function.

Are there therapeutic implications or potential targets related to CXCR4 in diseases involving immune dysregulation and inflammation?

There are potential therapeutic implications related to CXCR4 in diseases involving immune dysregulation and inflammation, making it a target for drug development.

How does CXCR4 contribute to the regulation of inflammatory responses and immune cell trafficking in various tissues?

CXCR4 contributes to the regulation of inflammatory responses and immune cell trafficking in various tissues, influencing the outcome of immune reactions.

What is the primary function of C-X-C chemokine receptor type 4 (CXCR4) in cellular processes and immune responses?

C-X-C chemokine receptor type 4 (CXCR4) plays a crucial role in cellular processes, primarily contributing to immune responses and cell migration.