Active Recombinant Human DAG1 Protein, His-tagged

• Cat.No.: DAG1-549H
• Product Overview: Recombinant Human DAG1 (Accession # NP_004384) Met1-Val749, fused with with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.The ED50 for this effect is typically 1.5 - 6.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human DAG1, His-tagged has a calculated MW of 67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit). In SDS-PAGE migrates as 65-90 kDa & 20 kDa, reducing conditions.
• Protein length: Met1-Val749
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) [ Homo sapiens ]
• Official Symbol: DAG1
• Synonyms: DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254
• Gene ID: 1605
• mRNA Refseq: NM_001165928
• Protein Refseq: NP_001159400
• MIM: 128239
• UniProt ID: Q14118
• Chromosome Location: 3p21

Reviews

08/17/2022

By utilizing the DAG1 protein in my experiments, I can trust that I am working with a product of the highest caliber, backed by a company dedicated to delivering exceptional quality and support.

01/23/2022

The reliability and reproducibility of the DAG1 Protein's performance make it a go-to option for WB experiments.

03/12/2021

The DAG1 protein is of exceptional quality and will undoubtedly meet my experimental needs with utmost reliability and accuracy.

Q&As

Are there any specific animal models used to study DAG1-related muscle disorders?

Yes, mouse models with mutations in the DAG1 gene are commonly used to study the pathophysiology of DAG1-related muscle disorders.

What are the symptoms of DAG1-related muscle disorders?

Symptoms can include muscle weakness, hypotonia, and developmental delay, as well as eye and brain abnormalities in some cases.

How does DAG1 protein contribute to the stability of muscle cell membranes?

DAG1 protein forms a complex with other proteins and glycoproteins to anchor the muscle cell membrane to the extracellular matrix.

Can DAG1 protein be used as a biomarker for muscle disorders?

Studies are exploring the potential use of DAG1 protein levels as a diagnostic and prognostic biomarker for muscle disorders.

How does DAG1 protein interact with other proteins in the muscle cell membrane?

DAG1 protein forms a complex with dystrophin, sarcoglycans, and other proteins to maintain the stability of the muscle cell membrane.