Active Recombinant Human DAPK1(1-296) Protein, N-GST-tagged

• Cat.No.: DAPK1-52H
• Product Overview: N-terminal GSTtagged, recombinant, human DAPK1, amino acids 1–296, expressed by baculovirus in Sf21 insect cells. Purified using glutathioneagarose.

Specification

• Description: Death-associated protein kinase 1 (DAPK1) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK1 modulates cell death induced by IFN-γ, TNF-α, Fas, and detachment from the extracellular matrix. DAPK1 has been also implicated in TGF-β-induced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK1.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-296 aa
• Form: 2.3mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 879U/mg, where one unit of DAPK1 activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM
• Molecular Mass: 60.9 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MTVFRQENVD 241 DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK SSRRGVSRED IEREVSILKE 301 IQHPNVITLH EVYENKTDVI LILELVAGGE LFDFLAEKES LTEEEATEFL KQILNGVYYL 361 HSLQIAHFDL KPENIMLLDR NVPKPRIKII DFGLAHKIDF GNEFKNIFGT PEFVAPEIVN 421 YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY FSNTSALAKD 481 FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKF
• Purity: Purity 97% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DAPK1 death associated protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death associated protein kinase 1; DAPK; ROCO3
• Gene ID: 1612
• mRNA Refseq: NM_001288729.2
• Protein Refseq: NP_001275658.1
• MIM: 600831
• UniProt ID: P53355

Reviews

12/15/2022

DAPK1 had the intended biological effect in the experiment.

04/11/2022

I've used many brands, but this one is one of my favorites.

03/17/2022

Good stability under different temperature and humidity conditions.

Q&As

What is the specific role of DAPK1 in apoptosis?

DAPK1 plays an important role in apoptosis, which can promote the process of apoptosis.

How can diseases be treated by regulating DAPK1 levels?

There are currently no drugs that directly regulate DAPK1 levels, but some DAPK1-related diseases can be treated by modulating its activity or regulating its expression.

What is the relationship of DAPK1 to other genes or proteins?

DAPK1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins to participate in a variety of biochemical reactions.

How to detect and analyze mutations in DAPK1?

Mutations in DAPK1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of DAPK1?

The types of mutations in DAPK1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

How to study the regulatory mechanism of DAPK1?

Studying the regulatory mechanism of DAPK1 requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.