Active Recombinant Human DAPK1, GST-tagged

• Cat.No.: DAPK1-1395H
• Product Overview: Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by γ-interferon. Activation of DAPK occurs via dephosphorylation of Ser-308 and subsequent association of calcium/calmodulin. DAPK is rapidly dephosphorylated in response to tumor necrosis factor or ceramide and then subsequently degraded via proteasome activity. The decline in DAPK expression is paralleled with increased caspase activity and cell apoptosis. Studies suggest that the apoptosis regulatory activities mediated by DAPK are controlled both by phosphorylation status and protein stability.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 49 nmol/min/mg
• Molecular Mass: ~71 kDa
• Protein length: 1-363 aa
• Purity: >80%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: DAPK1 death-associated protein kinase 1 [ Homo sapiens ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death-associated protein kinase 1; DAPK; DAP kinase 1; DKFZp781I035
• Gene ID: 1612
• mRNA Refseq: NM_004938
• Protein Refseq: NP_004929
• MIM: 600831
• UniProt ID: P53355
• Chromosome Location: 9q34.1
• Pathway: Apoptosis, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; IFN-gamma pathway, organism-specific biosystem; Pathways in cancer, organism-specific biosystem; Regulation of Apoptosis, organism-specific biosystem; Role of DCC in regulating apoptosis, organism-specific biosystem
• Function: ATP binding; calmodulin binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Reviews

12/15/2022

DAPK1 had the intended biological effect in the experiment.

04/11/2022

I've used many brands, but this one is one of my favorites.

03/17/2022

Good stability under different temperature and humidity conditions.

Q&As

What is the specific role of DAPK1 in apoptosis?

DAPK1 plays an important role in apoptosis, which can promote the process of apoptosis.

How can diseases be treated by regulating DAPK1 levels?

There are currently no drugs that directly regulate DAPK1 levels, but some DAPK1-related diseases can be treated by modulating its activity or regulating its expression.

What is the relationship of DAPK1 to other genes or proteins?

DAPK1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins to participate in a variety of biochemical reactions.

How to detect and analyze mutations in DAPK1?

Mutations in DAPK1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of DAPK1?

The types of mutations in DAPK1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

How to study the regulatory mechanism of DAPK1?

Studying the regulatory mechanism of DAPK1 requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.