Active Recombinant Human DAPK1 protein, His&GST-tagged

• Cat.No.: DAPK1-971H
• Product Overview: Recombinant Human DAPK1 protein(P53355-1)(Met1-Leu363), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
• Molecular Mass: The recombinant human DAPK1 (aa 1-363)/GST chimera consists of 600 amino acids and has a calculated molecular mass of 69.4 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Leu363
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DAPK1 death-associated protein kinase 1 [ Homo sapiens ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death-associated protein kinase 1; DAPK; DAP kinase 1; DKFZp781I035
• Gene ID: 1612
• mRNA Refseq: NM_004938
• Protein Refseq: NP_004929
• MIM: 600831
• UniProt ID: P53355

Reviews

12/15/2022

DAPK1 had the intended biological effect in the experiment.

04/11/2022

I've used many brands, but this one is one of my favorites.

03/17/2022

Good stability under different temperature and humidity conditions.

Q&As

What is the specific role of DAPK1 in apoptosis?

DAPK1 plays an important role in apoptosis, which can promote the process of apoptosis.

How can diseases be treated by regulating DAPK1 levels?

There are currently no drugs that directly regulate DAPK1 levels, but some DAPK1-related diseases can be treated by modulating its activity or regulating its expression.

What is the relationship of DAPK1 to other genes or proteins?

DAPK1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins to participate in a variety of biochemical reactions.

How to detect and analyze mutations in DAPK1?

Mutations in DAPK1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of DAPK1?

The types of mutations in DAPK1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

How to study the regulatory mechanism of DAPK1?

Studying the regulatory mechanism of DAPK1 requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.